FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2053201738> ?p ?o ?g. }

• W2053201738 endingPage "39" @default.
• W2053201738 startingPage "30" @default.
• W2053201738 abstract "Here, the role of methylation in regulation of rat Oct4 gene was evaluated during embryonic development, in adult tissues and in embryo-derived cells. First, the region 3.1 kb upstream to the rat Oct4 ATG site was cloned and sequenced. The rat Oct4 upstream sequence was similar to that in bovine, mouse and human with two upstream elements: proximal (PE) and distal enhancers (DE) and four homology conserved regions (CR1-4). The conserved regions in the rat have 69% - 96% homology with bovine, human, mouse sequences. Next, the methylation pattern in the promoter was determined during embryonic development, in adult tissues, in rat embryonic stem cell (ESC)-like cells and umbilical cord-derived cells (the feeder for ESC-like cells) using the bisulfite method and DNA sequencing. The promoter was methylated in adult and fetal tissues, and in days post coitus (DPC) 10.5 and 12.5 embryos and hypomethylated in DPC4.5 embryos and in rat ESC-like cells. The expression of Oct4 was evaluated by qRT-PCR. DPC4.5 embryos and rat ESC-like cells had higher expression of the Oct4 gene compared to DPC10.5 and 12.5 embryos, adult tissues and embryoid bodies derived from rat ESC-like cells. Thus, the methylation status correlated with the qRT-PCR results. These results indicate that the rat Oct4 3.1kb promoter region is organized and contains transcription binding and regulatory sites similar to those described for bovine, mouse and human. The rat Oct4 promoter is methylated during embryonic development after 4.5 DPC and during differentiation of rat ESC-like cells to embryoid bodies." @default.
• W2053201738 created "2016-06-24" @default.
• W2053201738 creator A5013005166 @default.
• W2053201738 creator A5025335310 @default.
• W2053201738 creator A5062740456 @default.
• W2053201738 creator A5070007619 @default.
• W2053201738 date "2009-05-11" @default.
• W2053201738 modified "2023-09-25" @default.
• W2053201738 title "Cloning and Characterization of 3.1kb Promoter Region of the Oct4 Gene from the Fischer 344 Rat" @default.
• W2053201738 cites W1594589613 @default.
• W2053201738 cites W1608762409 @default.
• W2053201738 cites W167532633 @default.
• W2053201738 cites W171108017 @default.
• W2053201738 cites W1927826942 @default.
• W2053201738 cites W1957763880 @default.
• W2053201738 cites W1964661730 @default.
• W2053201738 cites W1967064280 @default.
• W2053201738 cites W1968581446 @default.
• W2053201738 cites W1970528491 @default.
• W2053201738 cites W1972597600 @default.
• W2053201738 cites W1973340980 @default.
• W2053201738 cites W1973628413 @default.
• W2053201738 cites W1979981202 @default.
• W2053201738 cites W1981088384 @default.
• W2053201738 cites W1984470808 @default.
• W2053201738 cites W1988296009 @default.
• W2053201738 cites W1988805575 @default.
• W2053201738 cites W1990718295 @default.
• W2053201738 cites W1992525863 @default.
• W2053201738 cites W1993174854 @default.
• W2053201738 cites W1993709320 @default.
• W2053201738 cites W1995140011 @default.
• W2053201738 cites W2000033452 @default.
• W2053201738 cites W2000666750 @default.
• W2053201738 cites W2003529019 @default.
• W2053201738 cites W2005205452 @default.
• W2053201738 cites W2010031978 @default.
• W2053201738 cites W2013980278 @default.
• W2053201738 cites W2016161982 @default.
• W2053201738 cites W2026091217 @default.
• W2053201738 cites W2034293734 @default.
• W2053201738 cites W2034793893 @default.
• W2053201738 cites W2038526670 @default.
• W2053201738 cites W2052002403 @default.
• W2053201738 cites W2064679426 @default.
• W2053201738 cites W2065746091 @default.
• W2053201738 cites W2072846301 @default.
• W2053201738 cites W2075857997 @default.
• W2053201738 cites W2088514277 @default.
• W2053201738 cites W2096237918 @default.
• W2053201738 cites W2096592632 @default.
• W2053201738 cites W2100761325 @default.
• W2053201738 cites W2104708641 @default.
• W2053201738 cites W2107575419 @default.
• W2053201738 cites W2112485709 @default.
• W2053201738 cites W2115504899 @default.
• W2053201738 cites W2116205673 @default.
• W2053201738 cites W2123400162 @default.
• W2053201738 cites W2125987139 @default.
• W2053201738 cites W2127799088 @default.
• W2053201738 cites W2131068396 @default.
• W2053201738 cites W2132555347 @default.
• W2053201738 cites W2133990340 @default.
• W2053201738 cites W2138977668 @default.
• W2053201738 cites W2142046859 @default.
• W2053201738 cites W2149608498 @default.
• W2053201738 cites W2157985333 @default.
• W2053201738 cites W2160215297 @default.
• W2053201738 cites W2165518576 @default.
• W2053201738 cites W2167893559 @default.
• W2053201738 cites W74964901 @default.
• W2053201738 doi "https://doi.org/10.2174/1876893800901010030" @default.
• W2053201738 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3280817" @default.
• W2053201738 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22347989" @default.
• W2053201738 hasPublicationYear "2009" @default.
• W2053201738 type Work @default.
• W2053201738 sameAs 2053201738 @default.
• W2053201738 citedByCount "10" @default.
• W2053201738 countsByYear W20532017382012 @default.
• W2053201738 countsByYear W20532017382013 @default.
• W2053201738 countsByYear W20532017382016 @default.
• W2053201738 countsByYear W20532017382017 @default.
• W2053201738 countsByYear W20532017382018 @default.
• W2053201738 crossrefType "journal-article" @default.
• W2053201738 hasAuthorship W2053201738A5013005166 @default.
• W2053201738 hasAuthorship W2053201738A5025335310 @default.
• W2053201738 hasAuthorship W2053201738A5062740456 @default.
• W2053201738 hasAuthorship W2053201738A5070007619 @default.
• W2053201738 hasBestOaLocation W20532017382 @default.
• W2053201738 hasConcept C101762097 @default.
• W2053201738 hasConcept C104317684 @default.
• W2053201738 hasConcept C111936080 @default.
• W2053201738 hasConcept C121050878 @default.
• W2053201738 hasConcept C138885662 @default.
• W2053201738 hasConcept C143948831 @default.
• W2053201738 hasConcept C145103041 @default.
• W2053201738 hasConcept C150194340 @default.
• W2053201738 hasConcept C153911025 @default.

• next