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• W2053234014 startingPage "6708" @default.
• W2053234014 abstract "The quantitative study of noncovalent complexes by electrospray mass spectrometry requires the determination of the relative response of each species. The method proposed here to determine the electrospray response factors is based on the use of (1) an internal standard and (2) the mass balance equation applied to one binding partner M, for which different complexes MxLy are detected in the electrospray mass spectra. A set of experiments providing various ratios between the complexes (e.g., different ligand concentrations in a titration experiment or different time points in a kinetics experiment) is used to generate a set of independent linear equations that can be solved using simple matrix algebra to find the response factors of each MxLy complex relative to that of the internal standard. The response factors can then be used to determine equilibrium dissociation constants or for the quantitative monitoring of reaction kinetics. The first is illustrated with a study of DNA−ligand complexes, where we show that neither minor groove binding nor intercalation dramatically affects the DNA response factor. The second is illustrated with a study of the association kinetics of the telomeric G-quadruplex dGGG(TTAGGG)3 with its complementary strand, where the response factors allow correcting for the relative response of the quadruplex and the long duplex and obtaining reproducible association rate constants independently of the source tuning potentials." @default.
• W2053234014 created "2016-06-24" @default.
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• W2053234014 creator A5052261457 @default.
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• W2053234014 date "2009-07-10" @default.
• W2053234014 modified "2023-10-16" @default.
• W2053234014 title "A Simple Method to Determine Electrospray Response Factors of Noncovalent Complexes" @default.
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• W2053234014 doi "https://doi.org/10.1021/ac900785m" @default.
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