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- W2053240595 abstract "IN certain conditions bacterial initiation factors can discriminate between different classes of mRNA, or the individual cistrons of bacteriophage RNA1–5. Whether messenger specificity is a general phenomenon in eukaryotes as well is controversial, but several reports support the idea that mRNAs can be translated in heterologous systems without the addition of homologous tissue-specific factors, suggesting a lack of specificity6,10,17. These results have been obtained by adding mRNA either to oocytes7 or to crude cell-free systems such as lysates or 30,000g supernatants (S-30). With more fractionated systems—separated ribosomes reconstituted with cell sap—the existence of messenger specific factors is supported11–13. We have already demonstrated that an S-30 derived from either mouse or rat liver can faithfully translate rabbit globin mRNA without the addition of reticulocyte factors, and now report that fractionation of this S-30 using methods similar to those used by previous workers11–13 results in a system which will also translate globin mRNA. This fractionated system does not show a requirement for reticulocyte specific factors." @default.
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- W2053240595 date "1972-08-01" @default.
- W2053240595 modified "2023-09-25" @default.
- W2053240595 title "Translation of Rabbit Globin mRNA" @default.
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- W2053240595 doi "https://doi.org/10.1038/newbio238200a0" @default.
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