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- W2053241915 abstract "Poly(2-hydroxyethylmethacrylate-co-methacrylamidophenlyalanine) poly(HEMA-MAPA) membranes were prepared by UV-initiated photopolymerization of HEMA and MAPA. Lipase immobilization onto these membranes from aqueous solutions containing different amounts of lipase at different pH was investigated in a batch system. The lipase adsorption capacity of the membranes was increased as the MAPA ratio increased in the membrane structure. The maximum lipase immobilization capacity of the poly(HEMA-MAPA-3) membrane was 135 μg cm−2. The optimum temperature was 5°C higher than that of the free enzyme and was significantly broader. The storage stability increased with immobilization. The enzyme could be repeatedly adsorbed and desorbed without any significant loss in adsorption capacity." @default.
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- W2053241915 date "2001-03-01" @default.
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- W2053241915 title "Reversible immobilization of lipase on phenylalanine containing hydrogel membranes" @default.
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- W2053241915 doi "https://doi.org/10.1016/s0032-9592(00)00289-2" @default.
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