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- W2053262189 abstract "Background & Aims: The immunoregulatory properties of primary colonic epithelial cells (CECs) have not been defined. The ability of CECs from wild-type and interleukin 2–deficient (IL-2−/−) mice to take up a complex protein antigen and present peptides via MHC molecules to T cells was assessed and contrasted with that of primary small intestinal epithelial cells (SIECs). Methods: Uptake of fluorescein isothiocyanate (FITC)-labeled ovalbumin (FITC-OVA) by CECs and SIECs from wild-type and IL-2−/− mice was measured by flow cytometry. The effect of disrupting cytoskeleton organization and metabolic activity of CEC on antigen uptake was assessed. An OVA/I-Ab–specific CD4+ T-cell line transfected with an NFAT-lacZ reporter gene construct was used to evaluate the ability of CECs and SIECs as well as CECs from healthy and colitic IL-2−/− mice to present antigen to T cells. Results: Uptake of FITC-OVA by CECs is concentration dependent, is not saturated at physiologic concentrations, and requires metabolically active cells. CECs from IL-2−/− mice take up significantly more antigen than those from wild-type mice. CECs are more efficient APCs than SIECs, and antigen-pulsed CECs from IL-2−/− mice induce the highest levels of T-cell activation. Conclusions: Primary CECs are efficient APCs for CD4 MHC class II–restricted T cells. Antigen uptake and presentation is up-regulated in animals prone to develop intestinal inflammation.GASTROENTEROLOGY 2000;119:1548-1559" @default.
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- W2053262189 date "2000-12-01" @default.
- W2053262189 modified "2023-10-18" @default.
- W2053262189 title "Uptake and presentation of antigen to T cells by primary colonic epithelial cells in normal and diseased states" @default.
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- W2053262189 doi "https://doi.org/10.1053/gast.2000.20168" @default.
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