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- W2053274384 abstract "Hydrogen uptake by Azospirillum brasilense was studied in N 2 -fixing and NH 4 Cl-grown batch cultures. The K m for H 2 of uptake hydrogenase (O 2 -dependent H 3 H uptake) in whole cells was 9 µM. In N 2 -flxing or NH 4 Cl-grown cultures hydrogenase activity reached its maximum in late log phase, followed by a rapid decline in stationary phase. In cultures sparged with H 2 , hydrogenase activity was markedly prolonged. Rates of O 2 -dependent H 3 H uptake and H 2 uptake measured by gas chromatography were very similar. Hydrogenase derepression required microaerobic conditions, was independent of nitrogenase derepression, did not require exogenous H 2 , and may be enhanced by electron-donor limitation. Air caused irreversible inhibition of hydrogenase activity. In N-free cultures, theO 2 optima for H 2 uptake, ranging from 0.5 to 1.25% O 2 depending on the phase of growth, were significantly higher than those for nitrogenase activity (C 2 H 2 reduction), 0.15 to 0.35%, suggesting that H 2 uptake may have a limited ability to aid in the protection of nitrogenase against inactivation by O 2 ." @default.
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- W2053274384 date "1983-09-01" @default.
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- W2053274384 title "Effect of hydrogen and oxygen on uptake-hydrogenase activity in nitrogen-fixing and ammonium-grown Azospirillum brasilense" @default.
- W2053274384 doi "https://doi.org/10.1139/m83-172" @default.
- W2053274384 hasPublicationYear "1983" @default.
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