FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2053277504> ?p ?o ?g. }

• W2053277504 endingPage "4149" @default.
• W2053277504 startingPage "4141" @default.
• W2053277504 abstract "Epithelial cell adhesion molecule (EpCAM) is overexpressed in most solid cancers and is an ideal antigen for clinical applications in cancer diagnosis, prognosis, imaging, and therapy. Currently, most of the EpCAM-based diagnostic, prognostic, and therapeutic strategies rely on the anti-EpCAM antibody. However, the use of EpCAM antibody is restricted due to its large size and instability. In this study, we have successfully identified DNA aptamers that selectively bind human recombinant EpCAM protein. The aptamers can specifically recognize a number of live human cancer cells derived from breast, colorectal, and gastric cancers that express EpCAM but not bind to EpCAM-negative cells. Among the aptamer sequences identified, a hairpin-structured sequence SYL3 was optimized in length, resulting in aptamer sequence SYL3C. The Kd values of the SYL3C aptamer against breast cancer cell line MDA-MB-231 and gastric cancer cell line Kato III were found to be 38 ± 9 and 67 ± 8 nM, respectively, which are better than that of the full-length SYL3 aptamer. Flow cytometry analysis results indicated that the SYL3C aptamer was able to recognize target cancer cells from mixed cells in cell media. When used to capture cancer cells, up to 63% cancer cell capture efficiency was achieved with about 80% purity. With the advantages of small size, easy synthesis, good stability, high binding affinity, and selectivity, the DNA aptamers reported here against cancer biomarker EpCAM will facilitate the development of novel targeted cancer therapy, cancer cell imaging, and circulating tumor cell detection." @default.
• W2053277504 created "2016-06-24" @default.
• W2053277504 creator A5006349996 @default.
• W2053277504 creator A5025915904 @default.
• W2053277504 creator A5039084091 @default.
• W2053277504 creator A5040924293 @default.
• W2053277504 creator A5045338115 @default.
• W2053277504 creator A5049598065 @default.
• W2053277504 creator A5073050892 @default.
• W2053277504 creator A5080147219 @default.
• W2053277504 creator A5088285841 @default.
• W2053277504 date "2013-03-29" @default.
• W2053277504 modified "2023-10-16" @default.
• W2053277504 title "Selection of DNA Aptamers against Epithelial Cell Adhesion Molecule for Cancer Cell Imaging and Circulating Tumor Cell Capture" @default.
• W2053277504 cites W1940439262 @default.
• W2053277504 cites W1969894206 @default.
• W2053277504 cites W1971647685 @default.
• W2053277504 cites W1980657872 @default.
• W2053277504 cites W1987561076 @default.
• W2053277504 cites W1988835634 @default.
• W2053277504 cites W1990046017 @default.
• W2053277504 cites W1993585856 @default.
• W2053277504 cites W1997045968 @default.
• W2053277504 cites W1997686479 @default.
• W2053277504 cites W2000649281 @default.
• W2053277504 cites W2018334083 @default.
• W2053277504 cites W2020997268 @default.
• W2053277504 cites W2021110283 @default.
• W2053277504 cites W2034192753 @default.
• W2053277504 cites W2034491455 @default.
• W2053277504 cites W2037150116 @default.
• W2053277504 cites W2037534141 @default.
• W2053277504 cites W2038371089 @default.
• W2053277504 cites W2050547995 @default.
• W2053277504 cites W2050827942 @default.
• W2053277504 cites W2051572649 @default.
• W2053277504 cites W2052139810 @default.
• W2053277504 cites W2053588414 @default.
• W2053277504 cites W2055721693 @default.
• W2053277504 cites W2059281433 @default.
• W2053277504 cites W2063688529 @default.
• W2053277504 cites W2066074720 @default.
• W2053277504 cites W2073582321 @default.
• W2053277504 cites W2073883364 @default.
• W2053277504 cites W2074957872 @default.
• W2053277504 cites W2079321730 @default.
• W2053277504 cites W2082462273 @default.
• W2053277504 cites W2083558581 @default.
• W2053277504 cites W2091437725 @default.
• W2053277504 cites W2094564328 @default.
• W2053277504 cites W2100117577 @default.
• W2053277504 cites W2104092717 @default.
• W2053277504 cites W2104525153 @default.
• W2053277504 cites W2124258497 @default.
• W2053277504 cites W2125121305 @default.
• W2053277504 cites W2158654777 @default.
• W2053277504 cites W2158741313 @default.
• W2053277504 cites W2167448382 @default.
• W2053277504 cites W2167766248 @default.
• W2053277504 doi "https://doi.org/10.1021/ac400366b" @default.
• W2053277504 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23480100" @default.
• W2053277504 hasPublicationYear "2013" @default.
• W2053277504 type Work @default.
• W2053277504 sameAs 2053277504 @default.
• W2053277504 citedByCount "366" @default.
• W2053277504 countsByYear W20532775042013 @default.
• W2053277504 countsByYear W20532775042014 @default.
• W2053277504 countsByYear W20532775042015 @default.
• W2053277504 countsByYear W20532775042016 @default.
• W2053277504 countsByYear W20532775042017 @default.
• W2053277504 countsByYear W20532775042018 @default.
• W2053277504 countsByYear W20532775042019 @default.
• W2053277504 countsByYear W20532775042020 @default.
• W2053277504 countsByYear W20532775042021 @default.
• W2053277504 countsByYear W20532775042022 @default.
• W2053277504 countsByYear W20532775042023 @default.
• W2053277504 crossrefType "journal-article" @default.
• W2053277504 hasAuthorship W2053277504A5006349996 @default.
• W2053277504 hasAuthorship W2053277504A5025915904 @default.
• W2053277504 hasAuthorship W2053277504A5039084091 @default.
• W2053277504 hasAuthorship W2053277504A5040924293 @default.
• W2053277504 hasAuthorship W2053277504A5045338115 @default.
• W2053277504 hasAuthorship W2053277504A5049598065 @default.
• W2053277504 hasAuthorship W2053277504A5073050892 @default.
• W2053277504 hasAuthorship W2053277504A5080147219 @default.
• W2053277504 hasAuthorship W2053277504A5088285841 @default.
• W2053277504 hasConcept C121608353 @default.
• W2053277504 hasConcept C128972844 @default.
• W2053277504 hasConcept C1491633281 @default.
• W2053277504 hasConcept C153911025 @default.
• W2053277504 hasConcept C159654299 @default.
• W2053277504 hasConcept C185592680 @default.
• W2053277504 hasConcept C203014093 @default.
• W2053277504 hasConcept C2776677690 @default.
• W2053277504 hasConcept C502942594 @default.
• W2053277504 hasConcept C54355233 @default.
• W2053277504 hasConcept C553184892 @default.
• W2053277504 hasConcept C55493867 @default.

• next