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- W2053283592 abstract "The equilibrium behaviour of the bovine phosphatidylethanolamine-binding protein (PEBP) has been studied under various conditions of pH, temperature and urea concentration. Far-UV and near-UV CD, fluorescence and Fourier transform infrared spectroscopies indicate that, in its native state, PEBP is mainly composed of β-sheets, with Trp residues mostly localized in a hydrophobic environment; these results suggest that the conformation of PEBP in solution is similar to the three-dimensional structure determined by X-ray crystallography. The pH-induced conformational changes show a transition midpoint at pH 3.0, implying nine protons in the transition. At neutral pH, the thermal denaturation is irreversible due to protein precipitation, whereas at acidic pH values the protein exhibits a reversible denaturation. The thermal denaturation curves, as monitored by CD, fluorescence and differential scanning calorimetry, support a two-state model for the equilibrium and display coincident values with a melting temperature Tm = 54 °C, an enthalpy change ΔH = 119 kcal·mol−1 and a free energy change ΔG(H2O, 25 °C) = 5 kcal·mol−1. The urea-induced unfolding profiles of PEBP show a midpoint of the two-state unfolding transition at 4.8 m denaturant, and the stability of PEBP is 4.5 kcal·mol−1 at 25 °C. Moreover, the surface active properties indicate that PEBP is essentially a hydrophilic protein which progressively unfolds at the air/water interface over the course of time. Together, these results suggest that PEBP is well-structured in solution but that its conformation is weakly stable and sensitive to hydrophobic conditions: the PEBP structure seems to be flexible and adaptable to its environment." @default.
- W2053283592 created "2016-06-24" @default.
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- W2053283592 date "1999-11-15" @default.
- W2053283592 modified "2023-10-16" @default.
- W2053283592 title "Stability and physicochemical properties of the bovine brain phosphatidylethanolamine-binding protein" @default.
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- W2053283592 doi "https://doi.org/10.1046/j.1432-1327.1999.00812.x" @default.
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