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- W2053287395 abstract "Listeria Selective Isolation Agar (LSI) and Modified Acriflavin Ceftazidime Esculin Agar (MACE) were compared to McBride Listeria Agar minus Blood (MLA-B) for ability to recover Listeria monocytogenes Scott A cells inoculated into commerical yogurt, pH 4.1. Yogurt was stored at 5 ° C and sampled periodically over a 12 day period. LSI, MACE and MLA-B inhibited the growth of the two yogurt organisms but LSI and MACE gave better inhibition of other separately tested Gram-positive bacteria likely to be present in other fermented foods. Acid-stressed Listeria monocytogenes Scott A cells were optimally recovered by enrichment at 5 ° C for 5–18 days in 0.1M phosphate buffer, pH 7.2, followed by transfer to tryptic soy broth + 0.5% yeast extract at 30 ° C for 2 days. At low inoculum levels (10 2 cells/g yogurt), they were not detectable by direct plating or enrichment of yogurt after day 0. At high inoculum levels (10 7 cells/g yogurt), they were detectable up to day 6 but not at day 9 by direct plating on MLA-B, LSI or MACE with log counts per gram of yogurt being about 10 fold higher on LSI than on MACE or MLA-B. The above enrichment procedure permitted recovery on MLA-B, LSI, or MACE of viable Listeria cells from the day 9 samples found negative by direct plating." @default.
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- W2053287395 date "1988-10-01" @default.
- W2053287395 modified "2023-09-27" @default.
- W2053287395 title "Persistence of Listeria monocytogenes in yogurt as determined by direct plating and enrichment methods" @default.
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- W2053287395 doi "https://doi.org/10.1016/0168-1605(88)90007-4" @default.
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