Matches in SemOpenAlex for { <https://semopenalex.org/work/W2053288839> ?p ?o ?g. }
- W2053288839 endingPage "1648" @default.
- W2053288839 startingPage "1640" @default.
- W2053288839 abstract "Understanding the antibody response in botulinum intoxication is important for vaccine design and passive prophylaxis. To investigate this activity, we have studied the immune response to BoNT/A (botulinum neurotoxin serotype A) binding domain (HC) at the molecular level using phage display. The scFv antibodies were isolated from V-gene repertoires prepared from (a) human volunteer immunized with pentavalent botulinum toxoid and (b) non-immune human peripheral blood lymphocytes and spleenocytes. A large panel of serotype specific phage expressing botulinum binding scFv could be selected from both libraries. Epitope mapping of immune scFv binders towards BoNT/A HC revealed surprisingly a limited number of scFv recognizing conformational epitopes that corresponded to two distinct groups, clusters I and II. Only scFv from cluster I exhibited neutralizing activity in the mouse hemidiaphragm assay. Anti- BoNT/A HC clones derived from a non-immune library could be conveniently grouped into clusters III-XI and appeared to share no overlapping epitopes with cluster I or II. In addition they showed no neutralization of toxin at biologically significant concentrations. We therefore suggest that a vaccine based on the pentavalent botulinum toxoid directs the humoral immune response to a limited number of immunodominant epitopes exposed on the binding domain HC." @default.
- W2053288839 created "2016-06-24" @default.
- W2053288839 creator A5014251539 @default.
- W2053288839 creator A5032466026 @default.
- W2053288839 creator A5038349275 @default.
- W2053288839 creator A5041486049 @default.
- W2053288839 creator A5054336804 @default.
- W2053288839 creator A5063025236 @default.
- W2053288839 creator A5064447025 @default.
- W2053288839 date "2002-02-01" @default.
- W2053288839 modified "2023-09-23" @default.
- W2053288839 title "Genetic and immunological comparison of anti-botulinum type A antibodies from immune and non-immune human phage libraries" @default.
- W2053288839 cites W117988634 @default.
- W2053288839 cites W1531785323 @default.
- W2053288839 cites W1549258100 @default.
- W2053288839 cites W1927043766 @default.
- W2053288839 cites W1986493103 @default.
- W2053288839 cites W2007478374 @default.
- W2053288839 cites W2011261569 @default.
- W2053288839 cites W2013285346 @default.
- W2053288839 cites W2025827008 @default.
- W2053288839 cites W2034992208 @default.
- W2053288839 cites W2035826838 @default.
- W2053288839 cites W2041010702 @default.
- W2053288839 cites W2046981364 @default.
- W2053288839 cites W2054592480 @default.
- W2053288839 cites W2070777308 @default.
- W2053288839 cites W2075449190 @default.
- W2053288839 cites W2079912248 @default.
- W2053288839 cites W2081769761 @default.
- W2053288839 cites W2088986997 @default.
- W2053288839 cites W2089836311 @default.
- W2053288839 cites W2090997509 @default.
- W2053288839 cites W2096214890 @default.
- W2053288839 cites W2102074842 @default.
- W2053288839 cites W2106199484 @default.
- W2053288839 cites W2110495087 @default.
- W2053288839 cites W2123631149 @default.
- W2053288839 cites W2126444066 @default.
- W2053288839 cites W2133511513 @default.
- W2053288839 cites W2134886492 @default.
- W2053288839 cites W2138352514 @default.
- W2053288839 cites W2146159207 @default.
- W2053288839 cites W2154216619 @default.
- W2053288839 cites W2486899353 @default.
- W2053288839 cites W2897322349 @default.
- W2053288839 cites W4320301114 @default.
- W2053288839 doi "https://doi.org/10.1016/s0264-410x(01)00482-0" @default.
- W2053288839 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11858873" @default.
- W2053288839 hasPublicationYear "2002" @default.
- W2053288839 type Work @default.
- W2053288839 sameAs 2053288839 @default.
- W2053288839 citedByCount "91" @default.
- W2053288839 countsByYear W20532888392012 @default.
- W2053288839 countsByYear W20532888392013 @default.
- W2053288839 countsByYear W20532888392015 @default.
- W2053288839 countsByYear W20532888392016 @default.
- W2053288839 countsByYear W20532888392017 @default.
- W2053288839 countsByYear W20532888392019 @default.
- W2053288839 countsByYear W20532888392020 @default.
- W2053288839 countsByYear W20532888392021 @default.
- W2053288839 countsByYear W20532888392022 @default.
- W2053288839 crossrefType "journal-article" @default.
- W2053288839 hasAuthorship W2053288839A5014251539 @default.
- W2053288839 hasAuthorship W2053288839A5032466026 @default.
- W2053288839 hasAuthorship W2053288839A5038349275 @default.
- W2053288839 hasAuthorship W2053288839A5041486049 @default.
- W2053288839 hasAuthorship W2053288839A5054336804 @default.
- W2053288839 hasAuthorship W2053288839A5063025236 @default.
- W2053288839 hasAuthorship W2053288839A5064447025 @default.
- W2053288839 hasConcept C14086860 @default.
- W2053288839 hasConcept C153911025 @default.
- W2053288839 hasConcept C159047783 @default.
- W2053288839 hasConcept C159654299 @default.
- W2053288839 hasConcept C186268636 @default.
- W2053288839 hasConcept C195616568 @default.
- W2053288839 hasConcept C203014093 @default.
- W2053288839 hasConcept C2777010668 @default.
- W2053288839 hasConcept C2777351567 @default.
- W2053288839 hasConcept C2777367657 @default.
- W2053288839 hasConcept C2780801004 @default.
- W2053288839 hasConcept C2781241564 @default.
- W2053288839 hasConcept C86803240 @default.
- W2053288839 hasConcept C8891405 @default.
- W2053288839 hasConcept C89423630 @default.
- W2053288839 hasConceptScore W2053288839C14086860 @default.
- W2053288839 hasConceptScore W2053288839C153911025 @default.
- W2053288839 hasConceptScore W2053288839C159047783 @default.
- W2053288839 hasConceptScore W2053288839C159654299 @default.
- W2053288839 hasConceptScore W2053288839C186268636 @default.
- W2053288839 hasConceptScore W2053288839C195616568 @default.
- W2053288839 hasConceptScore W2053288839C203014093 @default.
- W2053288839 hasConceptScore W2053288839C2777010668 @default.
- W2053288839 hasConceptScore W2053288839C2777351567 @default.
- W2053288839 hasConceptScore W2053288839C2777367657 @default.
- W2053288839 hasConceptScore W2053288839C2780801004 @default.
- W2053288839 hasConceptScore W2053288839C2781241564 @default.
- W2053288839 hasConceptScore W2053288839C86803240 @default.