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- W2053289933 abstract "Cells of the green alga Selenastrum minutum display a high capacity for extra-mitochondrial O 2 consumption in the presence of effectors such as salicylhydroxamic acid and/or NADH. We provide evidence that this O 2 consumption is mediated by extracellular peroxidase. Peroxidase capacity, measured as the potential for stimulation of O 2 consumption by a combination of salicylhydroxamic acid and NADH, changed over a 10-day time course. Maximal stimulation of O 2 consumption occurred at day three, at which point the capacity for peroxidase-mediated O 2 consumption was three-to four-fold higher than that of the control O 2 consumption rate. Peroxidase-mediated O 2 consumption was sensitive to inhibition by 50 mM ascorbate and by cyanide. Cyanide titration curves indicated that O 2 consumption by peroxidase was much more sensitive to inhibition by cyanide than was O 2 consumption by cytochrome oxidase (I 50 < 1.6 μM and I 50 = 18.3 μM cyanide, respectively). By using evidence from a combination of cyanide titration curves and ascorbate inhibition, we concluded that despite a large capacity for peroxidase-mediated O 2 consumption, peroxidase did not measurably contribute to control rates of O 2 consumption. In the absence of effectors, O 2 consumption was mediated primarily by cytochrome oxidase." @default.
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- W2053289933 date "1996-02-01" @default.
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- W2053289933 title "Characterization of extracellular oxygen consumption by the green alga Selenastrum minutum" @default.
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- W2053289933 doi "https://doi.org/10.1111/j.1399-3054.1996.tb00213.x" @default.
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