Matches in SemOpenAlex for { <https://semopenalex.org/work/W2053343727> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W2053343727 endingPage "222" @default.
- W2053343727 startingPage "215" @default.
- W2053343727 abstract "Gene expression can be examined with different techniques including ribonuclease protection assay (RPA), in situ hybridisation (ISH), and quantitative reverse transcription-polymerase chain reaction (RT/PCR). These methods differ considerably in their sensitivity and precision in detecting and quantifying low abundance mRNA. Although there is evidence that RT/PCR can be performed in a quantitative manner, the quantitative capacity of this method is generally underestimated. To demonstrate that the comparative kinetic RT/PCR strategy-which uses a housekeeping gene as internal standard-is a quantitative method to detect significant differences in mRNA levels between different samples, the inhibitory effect of heparin on phorbol 12-myristate 13-acetate (PMA)-induced-TGF-beta1 mRNA expression was evaluated by RT/PCR and RPA, the standard method of mRNA quantification, and the results were compared. The reproducibility of RT/PCR amplification was calculated by comparing the quantity of G3PDH and TGF-beta1 PCR products, generated during the exponential phases, estimated from two different RT/PCR (G3PDH, r = 0.968, P = 0.0000; TGF-beta1, r = 0.966, P = 0.0000). The quantitative capacity of comparative kinetic RT/PCR was demonstrated by comparing the results obtained from RPA and RT/PCR using linear regression analysis. Starting from the same RNA extraction, but using only 1% of the RNA for the RT/PCR compared to RPA, significant correlation was observed (r = 0.984, P = 0.0004). Moreover the morphometric analysis of ISH signal was applied for the semi-quantitative evaluation of the expression and localisation of TGF-beta1 mRNA in the entire cell population. Our results demonstrate the close similarity of the RT/PCR and RPA methods in giving quantitative information on mRNA expression and indicate the possibility to adopt the comparative kinetic RT/PCR as reliable quantitative method of mRNA analysis." @default.
- W2053343727 created "2016-06-24" @default.
- W2053343727 creator A5000079750 @default.
- W2053343727 creator A5031992531 @default.
- W2053343727 creator A5041352440 @default.
- W2053343727 creator A5044135100 @default.
- W2053343727 creator A5049217502 @default.
- W2053343727 creator A5053346890 @default.
- W2053343727 creator A5073105208 @default.
- W2053343727 date "2001-01-01" @default.
- W2053343727 modified "2023-10-16" @default.
- W2053343727 title "Quantitation of TGF-?1 mRNA in porcine mesangial cells by comparative kinetic RT/PCR: Comparison with ribonuclease protection assay and in situ hybridization" @default.
- W2053343727 cites W1972985557 @default.
- W2053343727 cites W1982870383 @default.
- W2053343727 cites W1996139331 @default.
- W2053343727 cites W2001530093 @default.
- W2053343727 cites W2004312206 @default.
- W2053343727 cites W2022111740 @default.
- W2053343727 cites W2022133483 @default.
- W2053343727 cites W2056803129 @default.
- W2053343727 cites W2074599622 @default.
- W2053343727 cites W2093009120 @default.
- W2053343727 cites W2095352859 @default.
- W2053343727 cites W2120127794 @default.
- W2053343727 cites W2163284343 @default.
- W2053343727 cites W2414266694 @default.
- W2053343727 doi "https://doi.org/10.1002/jcla.1030" @default.
- W2053343727 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6807812" @default.
- W2053343727 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11436205" @default.
- W2053343727 hasPublicationYear "2001" @default.
- W2053343727 type Work @default.
- W2053343727 sameAs 2053343727 @default.
- W2053343727 citedByCount "19" @default.
- W2053343727 countsByYear W20533437272015 @default.
- W2053343727 countsByYear W20533437272023 @default.
- W2053343727 crossrefType "journal-article" @default.
- W2053343727 hasAuthorship W2053343727A5000079750 @default.
- W2053343727 hasAuthorship W2053343727A5031992531 @default.
- W2053343727 hasAuthorship W2053343727A5041352440 @default.
- W2053343727 hasAuthorship W2053343727A5044135100 @default.
- W2053343727 hasAuthorship W2053343727A5049217502 @default.
- W2053343727 hasAuthorship W2053343727A5053346890 @default.
- W2053343727 hasAuthorship W2053343727A5073105208 @default.
- W2053343727 hasBestOaLocation W20533437271 @default.
- W2053343727 hasConcept C104317684 @default.
- W2053343727 hasConcept C105580179 @default.
- W2053343727 hasConcept C150194340 @default.
- W2053343727 hasConcept C153911025 @default.
- W2053343727 hasConcept C156719811 @default.
- W2053343727 hasConcept C2778976237 @default.
- W2053343727 hasConcept C33922130 @default.
- W2053343727 hasConcept C4692481 @default.
- W2053343727 hasConcept C48023723 @default.
- W2053343727 hasConcept C55493867 @default.
- W2053343727 hasConcept C67705224 @default.
- W2053343727 hasConcept C86803240 @default.
- W2053343727 hasConceptScore W2053343727C104317684 @default.
- W2053343727 hasConceptScore W2053343727C105580179 @default.
- W2053343727 hasConceptScore W2053343727C150194340 @default.
- W2053343727 hasConceptScore W2053343727C153911025 @default.
- W2053343727 hasConceptScore W2053343727C156719811 @default.
- W2053343727 hasConceptScore W2053343727C2778976237 @default.
- W2053343727 hasConceptScore W2053343727C33922130 @default.
- W2053343727 hasConceptScore W2053343727C4692481 @default.
- W2053343727 hasConceptScore W2053343727C48023723 @default.
- W2053343727 hasConceptScore W2053343727C55493867 @default.
- W2053343727 hasConceptScore W2053343727C67705224 @default.
- W2053343727 hasConceptScore W2053343727C86803240 @default.
- W2053343727 hasIssue "4" @default.
- W2053343727 hasLocation W20533437271 @default.
- W2053343727 hasLocation W20533437272 @default.
- W2053343727 hasLocation W20533437273 @default.
- W2053343727 hasLocation W20533437274 @default.
- W2053343727 hasOpenAccess W2053343727 @default.
- W2053343727 hasPrimaryLocation W20533437271 @default.
- W2053343727 hasRelatedWork W175443584 @default.
- W2053343727 hasRelatedWork W2043338644 @default.
- W2053343727 hasRelatedWork W2151138684 @default.
- W2053343727 hasRelatedWork W2366222295 @default.
- W2053343727 hasRelatedWork W2378669086 @default.
- W2053343727 hasRelatedWork W2416218981 @default.
- W2053343727 hasRelatedWork W2943343189 @default.
- W2053343727 hasRelatedWork W3031220128 @default.
- W2053343727 hasRelatedWork W4303983510 @default.
- W2053343727 hasRelatedWork W3047931956 @default.
- W2053343727 hasVolume "15" @default.
- W2053343727 isParatext "false" @default.
- W2053343727 isRetracted "false" @default.
- W2053343727 magId "2053343727" @default.
- W2053343727 workType "article" @default.