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• W2053478049 startingPage "15272" @default.
• W2053478049 abstract "Agrobacterium tumefaciens can transfer part of its Ti plasmid, the T-DNA, to plant cells where it integrates into the nuclear genome via illegitimate recombination. Integration of the T-DNA results in small deletions of the plant target DNA, and may lead to truncation of the T-DNA borders and the production of filler DNA. We showed previously that T-DNA can also be transferred from A. tumefaciens to Sac-charomyces cerevisiae and integrates into the yeast genome via homologous recombination. We show here that when the T-DNA lacks homology with the S. cerevisiae genome, it integrates at random positions via illegitimate recombination. From 11 lines the integrated T-DNA was cloned back to Escherichia coli along with yeast flanking sequences. The T-DNA borders and yeast DNA flanking the T-DNA were sequenced and characterized. It was found that T-DNA integration had resulted in target DNA deletions and sometimes T-DNA truncations or filler DNA formation. Therefore, the molecular mechanism of illegitimate recombination by which T-DNA integrates in higher and lower eukaryotes seems conserved." @default.
• W2053478049 created "2016-06-24" @default.
• W2053478049 creator A5019958846 @default.
• W2053478049 creator A5074960830 @default.
• W2053478049 date "1996-12-24" @default.
• W2053478049 modified "2023-10-16" @default.
• W2053478049 title "Integration of <i>Agrobacterium tumefaciens</i> T-DNA in the <i>Saccharomyces cerevisiae</i> genome by illegitimate recombination" @default.
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• W2053478049 doi "https://doi.org/10.1073/pnas.93.26.15272" @default.
• W2053478049 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/26393" @default.
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