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• W2053492014 abstract "Here we describe a novel and efficient procedure for preparation of long uniform G4–DNA wires. The procedure includes (i) enzymatic synthesis of double-stranded DNA molecules consisting of long (up to 10,000 bases), continuous G strands and chains of complementary (dC)20-oligonucleotides, poly(dG)–n(dC)20; (ii) size exclusion HPLC separation of the G strands from the (dC)20 oligonucleotides in 0.1 M NaOH; and (iii) folding of the purified G strands into G4–DNA structures by lowering the pH to 7.0. We show by atomic force microscopy (AFM) that the preparation procedure yielded G4–DNA wires with a uniform morphology and a narrow length distribution. The correlation between the total amount of nucleotides in the G strands and the contour length of the G4–DNA molecules estimated by AFM suggests monomolecular folding of the G strands into quadruplex structures. The folding takes place either in the presence or in the absence of stabilizing ions (K+ or Na+). The addition of these cations leads to a dramatic change in the circular dichroism spectrum of the G4–DNA." @default.
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• W2053492014 date "2008-03-01" @default.
• W2053492014 modified "2023-10-18" @default.
• W2053492014 title "Efficient procedure of preparation and properties of long uniform G4–DNA nanowires" @default.
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• W2053492014 doi "https://doi.org/10.1016/j.ab.2007.10.017" @default.
• W2053492014 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17996714" @default.
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