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• W2053553043 abstract "Arcobacters are considered potential emerging food and waterborne pathogens. However, there is no data on the presence of Arcobacter spp. in fresh vegetables. Therefore the objective of this research was to study the presence of Arcobacter spp. in fresh lettuces. Fifty fresh lettuces purchased from different local shops in Valencia (Spain) were analyzed. The assay was performed simultaneously by cultural and molecular methods. Isolates were identified by real-time, multiplex PCR and restriction fragment length polymorphism analysis of PCR-amplified DNA fragment (PCR-RFLP). Finally, all the isolates were genotyped using the randomly amplified polymorphic DNA (RAPD-PCR) method. Arcobacter sp. was detected in 10 of the 50 samples (20%) by real-time PCR, being A. butzleri the unique detected species by mPCR. The detection levels obtained by conventional PCR (7 samples/50, 14%) were slightly lower. These seven samples were found to be positive also by culture isolation. All 19 obtained isolates were identified as A. butzleri by multiplex PCR and PCR-RFLP. Great genetic heterogeneity among the isolates was observed by RAPD-PCR profiling. To our knowledge, this is the first study in which Arcobacter spp. is detected in fresh vegetables such as lettuces. Although these foods are generally considered safe, given the large quantities consumed and the fact that further cooking is absent, lettuce could be a source of Arcobacters of public health concern." @default.
• W2053553043 created "2016-06-24" @default.
• W2053553043 creator A5053376821 @default.
• W2053553043 creator A5090195084 @default.
• W2053553043 date "2011-01-31" @default.
• W2053553043 modified "2023-09-30" @default.
• W2053553043 title "Study of Arcobacter spp. contamination in fresh lettuces detected by different cultural and molecular methods" @default.
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• W2053553043 doi "https://doi.org/10.1016/j.ijfoodmicro.2010.11.018" @default.
• W2053553043 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21167617" @default.
• W2053553043 hasPublicationYear "2011" @default.
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