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• W2053582912 endingPage "115802" @default.
• W2053582912 startingPage "115802" @default.
• W2053582912 abstract "Fast and sensitive cell viability identification is a key point for single cell analysis. To address this issue, this paper reports a novel single cell viability identification method based on the measurement of single cell shear adhesion force using an atomic force microscopy (AFM) cantilever-based micro putter. Viable and nonviable yeast cells are prepared and put onto three kinds of substrate surfaces, i.e. tungsten probe, gold and ITO substrate surfaces. A micro putter is fabricated from the AFM cantilever by focused ion beam etching technique. The spring constant of the micro putter is calibrated using the nanomanipulation approach. The shear adhesion force between the single viable or nonviable cell and each substrate is measured using the micro putter based on the nanorobotic manipulation system inside an environmental scanning electron microscope. The adhesion force is calculated based on the deflection of the micro putter beam. The results show that the adhesion force of the viable cell to the substrate is much larger than that of the nonviable cell. This identification method is label free, fast, sensitive and can give quantitative results at the single cell level." @default.
• W2053582912 created "2016-06-24" @default.
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• W2053582912 date "2011-10-05" @default.
• W2053582912 modified "2023-10-07" @default.
• W2053582912 title "Single cell adhesion force measurement for cell viability identification using an AFM cantilever-based micro putter" @default.
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• W2053582912 doi "https://doi.org/10.1088/0957-0233/22/11/115802" @default.
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