Matches in SemOpenAlex for { <https://semopenalex.org/work/W2053629316> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W2053629316 endingPage "472" @default.
- W2053629316 startingPage "467" @default.
- W2053629316 abstract "Mouse myeloma cells were transfected with pSV2-gpt and pSV2-neo based immunoglobulin expression vectors. Double transfectants were selected using the xanthine-guanine phosphoribosyl transferase (gpt) and the neomycin (neo) selection marker genes. A broad distribution in the level of mouse-human chimeric IgG expression was observed with series of independently isolated transfectoma clones. The relative amounts of secreted to membrane-bound antibodies correlated closely, which suggested, that fluorescence-activated cell sorting could be a valuable tool for the selection of high-yielding production cell lines. However, a single cycle of cell sorting did not steer the cloning process significantly toward cells that produce enhanced amounts of recombinant IgG. Only in cases in which the polyclonal transfectoma population contained a large percentage of nonproducing cells, these were successfully separated from the IgG-producing cell population. © 1996 John Wiley & Sons, Inc." @default.
- W2053629316 created "2016-06-24" @default.
- W2053629316 creator A5026314935 @default.
- W2053629316 creator A5060872094 @default.
- W2053629316 creator A5065537953 @default.
- W2053629316 creator A5084952747 @default.
- W2053629316 date "2000-03-26" @default.
- W2053629316 modified "2023-09-29" @default.
- W2053629316 title "Correlation between secreted and membrane-bound IgG in mouse myeloma cells transfected with chimeric immunoglobulin heavy and light chain genes" @default.
- W2053629316 cites W1492109500 @default.
- W2053629316 cites W1510526370 @default.
- W2053629316 cites W1971275366 @default.
- W2053629316 cites W1980826960 @default.
- W2053629316 cites W1980918464 @default.
- W2053629316 cites W2014095906 @default.
- W2053629316 cites W2038383400 @default.
- W2053629316 cites W2040027421 @default.
- W2053629316 cites W2042399661 @default.
- W2053629316 cites W2044019412 @default.
- W2053629316 cites W2054892173 @default.
- W2053629316 cites W2081707538 @default.
- W2053629316 cites W2085612220 @default.
- W2053629316 cites W2119007249 @default.
- W2053629316 cites W2127456956 @default.
- W2053629316 cites W2221403975 @default.
- W2053629316 doi "https://doi.org/10.1002/(sici)1097-0290(19960220)49:4<467::aid-bit14>3.0.co;2-7" @default.
- W2053629316 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18623602" @default.
- W2053629316 hasPublicationYear "2000" @default.
- W2053629316 type Work @default.
- W2053629316 sameAs 2053629316 @default.
- W2053629316 citedByCount "1" @default.
- W2053629316 crossrefType "journal-article" @default.
- W2053629316 hasAuthorship W2053629316A5026314935 @default.
- W2053629316 hasAuthorship W2053629316A5060872094 @default.
- W2053629316 hasAuthorship W2053629316A5065537953 @default.
- W2053629316 hasAuthorship W2053629316A5084952747 @default.
- W2053629316 hasConcept C104317684 @default.
- W2053629316 hasConcept C144024400 @default.
- W2053629316 hasConcept C149923435 @default.
- W2053629316 hasConcept C153911025 @default.
- W2053629316 hasConcept C159654299 @default.
- W2053629316 hasConcept C185592680 @default.
- W2053629316 hasConcept C203014093 @default.
- W2053629316 hasConcept C2778630268 @default.
- W2053629316 hasConcept C2908647359 @default.
- W2053629316 hasConcept C36394416 @default.
- W2053629316 hasConcept C40767141 @default.
- W2053629316 hasConcept C54009773 @default.
- W2053629316 hasConcept C54355233 @default.
- W2053629316 hasConcept C553184892 @default.
- W2053629316 hasConcept C55493867 @default.
- W2053629316 hasConcept C81885089 @default.
- W2053629316 hasConcept C86803240 @default.
- W2053629316 hasConcept C956191 @default.
- W2053629316 hasConcept C96007430 @default.
- W2053629316 hasConceptScore W2053629316C104317684 @default.
- W2053629316 hasConceptScore W2053629316C144024400 @default.
- W2053629316 hasConceptScore W2053629316C149923435 @default.
- W2053629316 hasConceptScore W2053629316C153911025 @default.
- W2053629316 hasConceptScore W2053629316C159654299 @default.
- W2053629316 hasConceptScore W2053629316C185592680 @default.
- W2053629316 hasConceptScore W2053629316C203014093 @default.
- W2053629316 hasConceptScore W2053629316C2778630268 @default.
- W2053629316 hasConceptScore W2053629316C2908647359 @default.
- W2053629316 hasConceptScore W2053629316C36394416 @default.
- W2053629316 hasConceptScore W2053629316C40767141 @default.
- W2053629316 hasConceptScore W2053629316C54009773 @default.
- W2053629316 hasConceptScore W2053629316C54355233 @default.
- W2053629316 hasConceptScore W2053629316C553184892 @default.
- W2053629316 hasConceptScore W2053629316C55493867 @default.
- W2053629316 hasConceptScore W2053629316C81885089 @default.
- W2053629316 hasConceptScore W2053629316C86803240 @default.
- W2053629316 hasConceptScore W2053629316C956191 @default.
- W2053629316 hasConceptScore W2053629316C96007430 @default.
- W2053629316 hasIssue "4" @default.
- W2053629316 hasLocation W20536293161 @default.
- W2053629316 hasLocation W20536293162 @default.
- W2053629316 hasOpenAccess W2053629316 @default.
- W2053629316 hasPrimaryLocation W20536293161 @default.
- W2053629316 hasRelatedWork W1567456473 @default.
- W2053629316 hasRelatedWork W1658725198 @default.
- W2053629316 hasRelatedWork W2348991363 @default.
- W2053629316 hasRelatedWork W2368739352 @default.
- W2053629316 hasRelatedWork W2371689295 @default.
- W2053629316 hasRelatedWork W2391446476 @default.
- W2053629316 hasRelatedWork W2404970265 @default.
- W2053629316 hasRelatedWork W2621347948 @default.
- W2053629316 hasRelatedWork W3026200651 @default.
- W2053629316 hasRelatedWork W3028649718 @default.
- W2053629316 hasVolume "49" @default.
- W2053629316 isParatext "false" @default.
- W2053629316 isRetracted "false" @default.
- W2053629316 magId "2053629316" @default.
- W2053629316 workType "article" @default.