Matches in SemOpenAlex for { <https://semopenalex.org/work/W2053643404> ?p ?o ?g. }
- W2053643404 endingPage "e39441" @default.
- W2053643404 startingPage "e39441" @default.
- W2053643404 abstract "Implantation is a complex process that requires a delicate cooperation between the immune and reproductive system. Any interference in the fine balance could result in embryo loss and infertility. We have recently shown that Toll-like receptor 5 activation results in a decrease of trophoblast cells binding to endometrial cells in an in vitro model of human implantation. However, little is known about the downstream signalling leading to the observed failure in implantation and the factors that modulate this immune response.An in vitro model of embryo implantation was used to evaluate the effect of trophoblasts and flagellin on the activation of NF-κB in endometrial cells and whether TLR5-related in vitro implantation failure is signalled through NF-κB. We generated two different NF-κB reporting cell lines by transfecting either an immortalized endometrial epithelial cell line (hTERT-EECs) or a human endometrial carcinoma cell line (Ishikawa 3-H-12) with a plasmid containing the secreted alkaline phosphatase (SEAP) under the control of five NF-κB sites. The presence of trophoblast cells as well as flagellin increased NF-κB activity when compared to controls. The NF-κB activation induced by flagellin was further increased by the addition of trophoblast cells. Moreover, blocking NF-κB signalling with a specific inhibitor (BAY11-7082) was able to restore the binding ability of our trophoblast cell line to the endometrial monolayer.These are the first results showing a local effect of the trophoblasts on the innate immune response of the endometrial epithelium. Moreover, we show that implantation failure caused by intrauterine infections could be associated with abnormal levels of NF-κB activation. Further studies are needed to evaluate the target genes through which NF-κB activation after TLR5 stimulation lead to failure in implantation and the effect of the embryo on those genes. Understanding these pathways could help in the diagnosis and treatment of implantation failure cases." @default.
- W2053643404 created "2016-06-24" @default.
- W2053643404 creator A5000793278 @default.
- W2053643404 creator A5001012483 @default.
- W2053643404 creator A5017218207 @default.
- W2053643404 creator A5018223123 @default.
- W2053643404 creator A5020216959 @default.
- W2053643404 creator A5021755720 @default.
- W2053643404 creator A5047925218 @default.
- W2053643404 creator A5082831716 @default.
- W2053643404 creator A5083430240 @default.
- W2053643404 date "2013-01-08" @default.
- W2053643404 modified "2023-10-18" @default.
- W2053643404 title "Human Trophoblast Cells Modulate Endometrial Cells Nuclear Factor κB Response to Flagellin In Vitro" @default.
- W2053643404 cites W1502651723 @default.
- W2053643404 cites W1539536848 @default.
- W2053643404 cites W1585083749 @default.
- W2053643404 cites W1655860314 @default.
- W2053643404 cites W1939915698 @default.
- W2053643404 cites W1981699226 @default.
- W2053643404 cites W1983419107 @default.
- W2053643404 cites W1993243592 @default.
- W2053643404 cites W1993697908 @default.
- W2053643404 cites W1993792235 @default.
- W2053643404 cites W1999177172 @default.
- W2053643404 cites W2001963326 @default.
- W2053643404 cites W2011091860 @default.
- W2053643404 cites W2015445169 @default.
- W2053643404 cites W2016056634 @default.
- W2053643404 cites W2019547929 @default.
- W2053643404 cites W2020240595 @default.
- W2053643404 cites W2027123878 @default.
- W2053643404 cites W2030200025 @default.
- W2053643404 cites W2037535156 @default.
- W2053643404 cites W203933131 @default.
- W2053643404 cites W2042851093 @default.
- W2053643404 cites W2053864622 @default.
- W2053643404 cites W2054420189 @default.
- W2053643404 cites W2058177024 @default.
- W2053643404 cites W2058922314 @default.
- W2053643404 cites W2068653484 @default.
- W2053643404 cites W2069755909 @default.
- W2053643404 cites W2074481738 @default.
- W2053643404 cites W2085519984 @default.
- W2053643404 cites W2098336582 @default.
- W2053643404 cites W2107797512 @default.
- W2053643404 cites W2113769407 @default.
- W2053643404 cites W2125682554 @default.
- W2053643404 cites W2137402023 @default.
- W2053643404 cites W2140455334 @default.
- W2053643404 cites W2143735061 @default.
- W2053643404 cites W2145444991 @default.
- W2053643404 cites W2146946844 @default.
- W2053643404 cites W2147763369 @default.
- W2053643404 cites W2156414024 @default.
- W2053643404 cites W2157223292 @default.
- W2053643404 cites W2157339124 @default.
- W2053643404 cites W2163316512 @default.
- W2053643404 cites W2165642605 @default.
- W2053643404 doi "https://doi.org/10.1371/journal.pone.0039441" @default.
- W2053643404 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3540055" @default.
- W2053643404 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23320062" @default.
- W2053643404 hasPublicationYear "2013" @default.
- W2053643404 type Work @default.
- W2053643404 sameAs 2053643404 @default.
- W2053643404 citedByCount "9" @default.
- W2053643404 countsByYear W20536434042015 @default.
- W2053643404 countsByYear W20536434042016 @default.
- W2053643404 countsByYear W20536434042017 @default.
- W2053643404 countsByYear W20536434042019 @default.
- W2053643404 countsByYear W20536434042020 @default.
- W2053643404 countsByYear W20536434042021 @default.
- W2053643404 countsByYear W20536434042022 @default.
- W2053643404 crossrefType "journal-article" @default.
- W2053643404 hasAuthorship W2053643404A5000793278 @default.
- W2053643404 hasAuthorship W2053643404A5001012483 @default.
- W2053643404 hasAuthorship W2053643404A5017218207 @default.
- W2053643404 hasAuthorship W2053643404A5018223123 @default.
- W2053643404 hasAuthorship W2053643404A5020216959 @default.
- W2053643404 hasAuthorship W2053643404A5021755720 @default.
- W2053643404 hasAuthorship W2053643404A5047925218 @default.
- W2053643404 hasAuthorship W2053643404A5082831716 @default.
- W2053643404 hasAuthorship W2053643404A5083430240 @default.
- W2053643404 hasBestOaLocation W20536434041 @default.
- W2053643404 hasConcept C170493617 @default.
- W2053643404 hasConcept C172680121 @default.
- W2053643404 hasConcept C203014093 @default.
- W2053643404 hasConcept C2776953305 @default.
- W2053643404 hasConcept C2777495146 @default.
- W2053643404 hasConcept C2779234561 @default.
- W2053643404 hasConcept C38117629 @default.
- W2053643404 hasConcept C54355233 @default.
- W2053643404 hasConcept C55493867 @default.
- W2053643404 hasConcept C6045943 @default.
- W2053643404 hasConcept C81885089 @default.
- W2053643404 hasConcept C86803240 @default.
- W2053643404 hasConcept C8891405 @default.
- W2053643404 hasConcept C95444343 @default.