FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2053660147> ?p ?o ?g. }

• W2053660147 endingPage "103" @default.
• W2053660147 startingPage "95" @default.
• W2053660147 abstract "(Na+ + K+)-ATPase activity of a dog kidney enzyme preparation was markedly inhibited by 10-30% (v/v) dimethyl sulfoxide (Me2SO) and ethylene glycol (Et(OH)2); moreover, Me2SO produced a pattern of uncompetitive inhibition toward ATP. However, K+-nitrophenylphosphatase activity was stimulated by 10-20% Me2SO and Et(OH)2 but was inhibited by 30-50%. Me2SO decreased the Km for this substrate but had little effect on the Vmax below 30% (at which concentration Vmax was then reduced). Me2SO also reduced the Ki for Pi and acetyl phosphate as competitors toward nitrophenyl phosphate but increased the Ki for ATP, CTP and 2-O-methylfluorescein phosphate as competitors. Me2SO inhibited K+-acetylphosphatase activity, although it also reduced the Km for that substrate. Finally, Me2SO increased the rate of enzyme inactivation by fluoride and beryllium. These observations are interpreted in terms of the E1P to E2P transition of the reaction sequence being associated with an increased hydrophobicity of the active site, and of Me2SO mimicking such effects by decreasing water activity: (i) primarily to stabilize the covalent E2P intermediate, through differential solvation of reactants and products, and thereby inhibiting the (Na+ + K+)-ATPase reaction and acting as a dead-end inhibitor to produce the pattern of uncompetitive inhibition; inhibiting the K+-acetylphosphatase reaction that also passes through an E2P intermediate; but not inhibiting (at lower Me2SO concentrations) the K+-nitrophenylphosphatase reaction that does not pass through such an intermediate; and (ii) secondarily to favor partitioning of Pi and non-nucleotide phosphates into the hydrophobic active site, thereby decreasing the Km for nitrophenyl phosphate and acetyl phosphate, the Ki for Pi and acetyl phosphate in the K+-nitrophenylphosphatase reaction, accelerating inactivation by fluoride and beryllium acting as phosphate analogs, and, at higher concentrations, inhibiting the K+-nitrophenylphosphatase reaction by stabilizing the non-covalent E2.P intermediate of that reaction. In addition, Me2SO may decrease binding at the adenine pocket of the low-affinity substrate site, represented as an increased Ki for ATP, CTP and 3-O-methylfluorescein phosphate." @default.
• W2053660147 created "2016-06-24" @default.
• W2053660147 creator A5056063788 @default.
• W2053660147 date "1989-02-01" @default.
• W2053660147 modified "2023-09-23" @default.
• W2053660147 title "Solvent effects on substrate and phosphate interactions with the (Na+ + K+)-ATPase" @default.
• W2053660147 cites W1014197125 @default.
• W2053660147 cites W1486839213 @default.
• W2053660147 cites W1492452850 @default.
• W2053660147 cites W1512646890 @default.
• W2053660147 cites W1524085286 @default.
• W2053660147 cites W1526835946 @default.
• W2053660147 cites W1554161190 @default.
• W2053660147 cites W1597136252 @default.
• W2053660147 cites W1657809798 @default.
• W2053660147 cites W1972122807 @default.
• W2053660147 cites W1977696012 @default.
• W2053660147 cites W1983863748 @default.
• W2053660147 cites W1985856832 @default.
• W2053660147 cites W1994865013 @default.
• W2053660147 cites W2004963309 @default.
• W2053660147 cites W2014060135 @default.
• W2053660147 cites W2016626455 @default.
• W2053660147 cites W2019471978 @default.
• W2053660147 cites W2025578817 @default.
• W2053660147 cites W2032796934 @default.
• W2053660147 cites W2042326757 @default.
• W2053660147 cites W2046301194 @default.
• W2053660147 cites W2055408986 @default.
• W2053660147 cites W2063404335 @default.
• W2053660147 cites W2065099034 @default.
• W2053660147 cites W2065543058 @default.
• W2053660147 cites W2066663992 @default.
• W2053660147 cites W2080057691 @default.
• W2053660147 cites W2084780288 @default.
• W2053660147 cites W2088839366 @default.
• W2053660147 cites W2129635086 @default.
• W2053660147 cites W2313922621 @default.
• W2053660147 cites W2462949057 @default.
• W2053660147 cites W36684287 @default.
• W2053660147 doi "https://doi.org/10.1016/0167-4838(89)90148-9" @default.
• W2053660147 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2535941" @default.
• W2053660147 hasPublicationYear "1989" @default.
• W2053660147 type Work @default.
• W2053660147 sameAs 2053660147 @default.
• W2053660147 citedByCount "13" @default.
• W2053660147 countsByYear W20536601472015 @default.
• W2053660147 crossrefType "journal-article" @default.
• W2053660147 hasAuthorship W2053660147A5056063788 @default.
• W2053660147 hasConcept C14728290 @default.
• W2053660147 hasConcept C150757390 @default.
• W2053660147 hasConcept C155647269 @default.
• W2053660147 hasConcept C178790620 @default.
• W2053660147 hasConcept C181199279 @default.
• W2053660147 hasConcept C185592680 @default.
• W2053660147 hasConcept C18903297 @default.
• W2053660147 hasConcept C2777132085 @default.
• W2053660147 hasConcept C2777289219 @default.
• W2053660147 hasConcept C2778452553 @default.
• W2053660147 hasConcept C41183919 @default.
• W2053660147 hasConcept C55493867 @default.
• W2053660147 hasConcept C71240020 @default.
• W2053660147 hasConcept C86803240 @default.
• W2053660147 hasConceptScore W2053660147C14728290 @default.
• W2053660147 hasConceptScore W2053660147C150757390 @default.
• W2053660147 hasConceptScore W2053660147C155647269 @default.
• W2053660147 hasConceptScore W2053660147C178790620 @default.
• W2053660147 hasConceptScore W2053660147C181199279 @default.
• W2053660147 hasConceptScore W2053660147C185592680 @default.
• W2053660147 hasConceptScore W2053660147C18903297 @default.
• W2053660147 hasConceptScore W2053660147C2777132085 @default.
• W2053660147 hasConceptScore W2053660147C2777289219 @default.
• W2053660147 hasConceptScore W2053660147C2778452553 @default.
• W2053660147 hasConceptScore W2053660147C41183919 @default.
• W2053660147 hasConceptScore W2053660147C55493867 @default.
• W2053660147 hasConceptScore W2053660147C71240020 @default.
• W2053660147 hasConceptScore W2053660147C86803240 @default.
• W2053660147 hasIssue "2" @default.
• W2053660147 hasLocation W20536601471 @default.
• W2053660147 hasLocation W20536601472 @default.
• W2053660147 hasOpenAccess W2053660147 @default.
• W2053660147 hasPrimaryLocation W20536601471 @default.
• W2053660147 hasRelatedWork W1563999032 @default.
• W2053660147 hasRelatedWork W1990066813 @default.
• W2053660147 hasRelatedWork W2056362934 @default.
• W2053660147 hasRelatedWork W2094136229 @default.
• W2053660147 hasRelatedWork W2286339685 @default.
• W2053660147 hasRelatedWork W2421508457 @default.
• W2053660147 hasRelatedWork W2994475855 @default.
• W2053660147 hasRelatedWork W3111370901 @default.
• W2053660147 hasRelatedWork W4285602645 @default.
• W2053660147 hasRelatedWork W4285605806 @default.
• W2053660147 hasVolume "994" @default.
• W2053660147 isParatext "false" @default.
• W2053660147 isRetracted "false" @default.
• W2053660147 magId "2053660147" @default.
• W2053660147 workType "article" @default.