FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2053695273> ?p ?o ?g. }

• W2053695273 endingPage "249" @default.
• W2053695273 startingPage "243" @default.
• W2053695273 abstract "The major constituent of the paired helical filaments (PHFs) of Alzheimer's disease is the abnormally phosphorylated form of the microtubule-associated protein, τ. Monoclonal antibody (mAb) Tau-1 is used extensively to stain normal human τ, and τ isolated from the brains of Alzheimer's disease patients after dephosphorylation. We used a panel of 6 synthetic peptides to localize the minimal epitope of Tau-1 between amino acids 192–204. All 4 serine residues within this fragment were later phosphorylated individually by chemical methods, and it was shown that none of the peptides carrying a single phosphate group were recognized by the antibody. The serines included those that are probably not transformed in AD and consequently, conclusions drawn about malfunctioning kinase activity, based on Tau-1 immunoreactivity, can be extremely misleading. The recognition was restored at a decreased level when one of the serines was replaced by an alanine residue. mAb AT8 was made by immunizing with the PHFs and was reported to recognize the same region of the protein in a phosphorylated form. AT8 did not, however, cross-react with any of the singly phosphorylated peptides, indicating that the recognition site of the two antibodies are not entirely complementary or the binding to AT8 needs multiple phosphorylation of the antigen. The abolished recognition of the phosphorylated peptides cannot be attributed to a conformational change due to phosphorylation, since all peptides exhibited reverse-turn secondary structures, as indicated by circular dichroism (CD) spectroscopy. Ant-τ mAbs may distinguish between phosphorylated and non-phosphorylated forms of epitopes regardless of the location of the phosphate group. © 1993 Wiley-Liss, Inc." @default.
• W2053695273 created "2016-06-24" @default.
• W2053695273 creator A5040450948 @default.
• W2053695273 creator A5063994917 @default.
• W2053695273 creator A5064458568 @default.
• W2053695273 date "1993-02-01" @default.
• W2053695273 modified "2023-10-17" @default.
• W2053695273 title "Recognition of the minimal epitope of monoclonal antibody Tau-1 depends upon the presence of a phosphate group but not its location" @default.
• W2053695273 cites W126426573 @default.
• W2053695273 cites W1515458173 @default.
• W2053695273 cites W1539339671 @default.
• W2053695273 cites W1587655337 @default.
• W2053695273 cites W1964886907 @default.
• W2053695273 cites W1967024359 @default.
• W2053695273 cites W1978066124 @default.
• W2053695273 cites W1984816958 @default.
• W2053695273 cites W1990379890 @default.
• W2053695273 cites W1994030229 @default.
• W2053695273 cites W1995515835 @default.
• W2053695273 cites W2017122640 @default.
• W2053695273 cites W2020805094 @default.
• W2053695273 cites W2036026294 @default.
• W2053695273 cites W2036755018 @default.
• W2053695273 cites W2039282729 @default.
• W2053695273 cites W2048379819 @default.
• W2053695273 cites W2060792213 @default.
• W2053695273 cites W2069734380 @default.
• W2053695273 cites W2072578069 @default.
• W2053695273 cites W2077422298 @default.
• W2053695273 cites W2090547876 @default.
• W2053695273 cites W2123926494 @default.
• W2053695273 cites W2159377066 @default.
• W2053695273 cites W2167241481 @default.
• W2053695273 cites W253655842 @default.
• W2053695273 doi "https://doi.org/10.1002/jnr.490340212" @default.
• W2053695273 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7680727" @default.
• W2053695273 hasPublicationYear "1993" @default.
• W2053695273 type Work @default.
• W2053695273 sameAs 2053695273 @default.
• W2053695273 citedByCount "186" @default.
• W2053695273 countsByYear W20536952732012 @default.
• W2053695273 countsByYear W20536952732013 @default.
• W2053695273 countsByYear W20536952732015 @default.
• W2053695273 countsByYear W20536952732016 @default.
• W2053695273 countsByYear W20536952732017 @default.
• W2053695273 countsByYear W20536952732018 @default.
• W2053695273 countsByYear W20536952732019 @default.
• W2053695273 countsByYear W20536952732020 @default.
• W2053695273 countsByYear W20536952732021 @default.
• W2053695273 countsByYear W20536952732022 @default.
• W2053695273 countsByYear W20536952732023 @default.
• W2053695273 crossrefType "journal-article" @default.
• W2053695273 hasAuthorship W2053695273A5040450948 @default.
• W2053695273 hasAuthorship W2053695273A5063994917 @default.
• W2053695273 hasAuthorship W2053695273A5064458568 @default.
• W2053695273 hasConcept C11960822 @default.
• W2053695273 hasConcept C133571119 @default.
• W2053695273 hasConcept C153911025 @default.
• W2053695273 hasConcept C159654299 @default.
• W2053695273 hasConcept C178666793 @default.
• W2053695273 hasConcept C181912034 @default.
• W2053695273 hasConcept C185592680 @default.
• W2053695273 hasConcept C195616568 @default.
• W2053695273 hasConcept C203014093 @default.
• W2053695273 hasConcept C2776414213 @default.
• W2053695273 hasConcept C2779281246 @default.
• W2053695273 hasConcept C2780632150 @default.
• W2053695273 hasConcept C542903549 @default.
• W2053695273 hasConcept C55493867 @default.
• W2053695273 hasConcept C86803240 @default.
• W2053695273 hasConceptScore W2053695273C11960822 @default.
• W2053695273 hasConceptScore W2053695273C133571119 @default.
• W2053695273 hasConceptScore W2053695273C153911025 @default.
• W2053695273 hasConceptScore W2053695273C159654299 @default.
• W2053695273 hasConceptScore W2053695273C178666793 @default.
• W2053695273 hasConceptScore W2053695273C181912034 @default.
• W2053695273 hasConceptScore W2053695273C185592680 @default.
• W2053695273 hasConceptScore W2053695273C195616568 @default.
• W2053695273 hasConceptScore W2053695273C203014093 @default.
• W2053695273 hasConceptScore W2053695273C2776414213 @default.
• W2053695273 hasConceptScore W2053695273C2779281246 @default.
• W2053695273 hasConceptScore W2053695273C2780632150 @default.
• W2053695273 hasConceptScore W2053695273C542903549 @default.
• W2053695273 hasConceptScore W2053695273C55493867 @default.
• W2053695273 hasConceptScore W2053695273C86803240 @default.
• W2053695273 hasIssue "2" @default.
• W2053695273 hasLocation W20536952731 @default.
• W2053695273 hasLocation W20536952732 @default.
• W2053695273 hasOpenAccess W2053695273 @default.
• W2053695273 hasPrimaryLocation W20536952731 @default.
• W2053695273 hasRelatedWork W1981898410 @default.
• W2053695273 hasRelatedWork W1982649822 @default.
• W2053695273 hasRelatedWork W2057779043 @default.
• W2053695273 hasRelatedWork W2091594406 @default.
• W2053695273 hasRelatedWork W2109572947 @default.
• W2053695273 hasRelatedWork W2125033869 @default.
• W2053695273 hasRelatedWork W2973574133 @default.
• W2053695273 hasRelatedWork W3105097885 @default.

• next