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- W2053752451 abstract "Abstract This paper describes a method for assigning temperature-sensitive ( ts ) mutations of influenza viruses to individual segments of the genome. Two strains of the avian influenza A virus fowl plague (Rostock and Dobson) have been used, because the individual RNA segments of each strain and their corresponding gene products can be distinguished by polyacrylamide gel electrophoresis. The method consists of generating a series of recombinants from mixed infection with ts mutants of both strains, and comparing the electrophoretic migration of the RNA and proteins of the recombinants with those of the parents. In the example presented here, the recombinants have been obtained from crosses involving the ts 47 mutant of Rostock and ultraviolet-irradiated ts i mutant of Dobson. It is shown that the ts lesion of the Rostock mutant occurs in the smallest gene (gene 8), whose corresponding gene product is the nonstructural (NS) protein. In addition, the genes coding for the nucleoprotein (NP) of both Rostock and Dobson strains have been identified." @default.
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- W2053752451 date "1977-08-01" @default.
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- W2053752451 title "Method for assigning temperature-sensitive mutations of influenza viruses to individual segments of the genome" @default.
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- W2053752451 doi "https://doi.org/10.1016/0042-6822(77)90058-7" @default.
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