Matches in SemOpenAlex for { <https://semopenalex.org/work/W2053800551> ?p ?o ?g. }
- W2053800551 endingPage "261" @default.
- W2053800551 startingPage "250" @default.
- W2053800551 abstract "ABSTRACT Us3 is a serine-threonine protein kinase encoded by herpes simplex virus 1 (HSV-1). As reported here, we attempted to identify the previously unreported physiological substrate of Us3 in HSV-1-infected cells. Our results were as follows. (i) Bioinformatics analysis predicted two putative Us3 phosphorylation sites in the viral envelope glycoprotein B (gB) at codons 557 to 562 (RRVSAR) and codons 884 to 889 (RRNTNY). (ii) In in vitro kinase assays, the threonine residue at position 887 (Thr-887) in the gB domain was specifically phosphorylated by Us3, while the serine residue at position 560 was not. (iii) The phosphorylation of gB Thr-887 in Vero cells infected with wild-type HSV-1 was specifically detected using an antibody that recognized phosphorylated serine or threonine residues with arginine at the −3 and −2 positions. (iv) The phosphorylation of gB Thr-887 in infected cells was dependent on the kinase activity of Us3. (v) The replacement of Thr-887 with alanine markedly upregulated the cell surface expression of gB in infected cells, whereas replacement with aspartic acid, which sometimes mimics constitutive phosphorylation, restored the wild-type phenotype. The upregulation of gB expression on the cell surface also was observed in cells infected with a recombinant HSV-1 encoding catalytically inactive Us3. These results supported the hypothesis that Us3 phosphorylates gB and downregulates the cell surface expression of gB in HSV-1-infected cells." @default.
- W2053800551 created "2016-06-24" @default.
- W2053800551 creator A5005243857 @default.
- W2053800551 creator A5033908480 @default.
- W2053800551 creator A5061556330 @default.
- W2053800551 creator A5062366510 @default.
- W2053800551 creator A5063905419 @default.
- W2053800551 creator A5091421622 @default.
- W2053800551 date "2009-01-01" @default.
- W2053800551 modified "2023-10-01" @default.
- W2053800551 title "Herpes Simplex Virus 1 Protein Kinase Us3 Phosphorylates Viral Envelope Glycoprotein B and Regulates Its Expression on the Cell Surface" @default.
- W2053800551 cites W1538343422 @default.
- W2053800551 cites W1554292695 @default.
- W2053800551 cites W1608386732 @default.
- W2053800551 cites W1912303405 @default.
- W2053800551 cites W1964440626 @default.
- W2053800551 cites W1970010476 @default.
- W2053800551 cites W1971663674 @default.
- W2053800551 cites W1978968762 @default.
- W2053800551 cites W1982655562 @default.
- W2053800551 cites W1986791214 @default.
- W2053800551 cites W1992512657 @default.
- W2053800551 cites W1993881377 @default.
- W2053800551 cites W1997221411 @default.
- W2053800551 cites W2001371234 @default.
- W2053800551 cites W2004668555 @default.
- W2053800551 cites W2004917770 @default.
- W2053800551 cites W2005210883 @default.
- W2053800551 cites W2006519535 @default.
- W2053800551 cites W2019395700 @default.
- W2053800551 cites W2025755348 @default.
- W2053800551 cites W2030205108 @default.
- W2053800551 cites W2031584044 @default.
- W2053800551 cites W2035109828 @default.
- W2053800551 cites W2042144075 @default.
- W2053800551 cites W2056897525 @default.
- W2053800551 cites W2067498099 @default.
- W2053800551 cites W2068592712 @default.
- W2053800551 cites W2078891065 @default.
- W2053800551 cites W2082779452 @default.
- W2053800551 cites W2095169342 @default.
- W2053800551 cites W2096130202 @default.
- W2053800551 cites W2096236582 @default.
- W2053800551 cites W2109128688 @default.
- W2053800551 cites W2114990920 @default.
- W2053800551 cites W2116877629 @default.
- W2053800551 cites W2117681698 @default.
- W2053800551 cites W2130056219 @default.
- W2053800551 cites W2132862837 @default.
- W2053800551 cites W2133575147 @default.
- W2053800551 cites W2135433503 @default.
- W2053800551 cites W2135814779 @default.
- W2053800551 cites W2138357022 @default.
- W2053800551 cites W2138395245 @default.
- W2053800551 cites W2138630631 @default.
- W2053800551 cites W2140225836 @default.
- W2053800551 cites W2142483675 @default.
- W2053800551 cites W2146470087 @default.
- W2053800551 cites W2147469769 @default.
- W2053800551 cites W2147881941 @default.
- W2053800551 cites W2148351021 @default.
- W2053800551 cites W2152170709 @default.
- W2053800551 cites W2153163492 @default.
- W2053800551 cites W2155026265 @default.
- W2053800551 cites W2156805103 @default.
- W2053800551 cites W2157856815 @default.
- W2053800551 cites W2158067882 @default.
- W2053800551 cites W2159293959 @default.
- W2053800551 cites W2162557708 @default.
- W2053800551 cites W2163598864 @default.
- W2053800551 cites W4229510242 @default.
- W2053800551 cites W4254750003 @default.
- W2053800551 doi "https://doi.org/10.1128/jvi.01451-08" @default.
- W2053800551 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2612323" @default.
- W2053800551 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18945776" @default.
- W2053800551 hasPublicationYear "2009" @default.
- W2053800551 type Work @default.
- W2053800551 sameAs 2053800551 @default.
- W2053800551 citedByCount "80" @default.
- W2053800551 countsByYear W20538005512013 @default.
- W2053800551 countsByYear W20538005512014 @default.
- W2053800551 countsByYear W20538005512015 @default.
- W2053800551 countsByYear W20538005512016 @default.
- W2053800551 countsByYear W20538005512017 @default.
- W2053800551 countsByYear W20538005512018 @default.
- W2053800551 countsByYear W20538005512019 @default.
- W2053800551 countsByYear W20538005512020 @default.
- W2053800551 countsByYear W20538005512021 @default.
- W2053800551 countsByYear W20538005512022 @default.
- W2053800551 countsByYear W20538005512023 @default.
- W2053800551 crossrefType "journal-article" @default.
- W2053800551 hasAuthorship W2053800551A5005243857 @default.
- W2053800551 hasAuthorship W2053800551A5033908480 @default.
- W2053800551 hasAuthorship W2053800551A5061556330 @default.
- W2053800551 hasAuthorship W2053800551A5062366510 @default.
- W2053800551 hasAuthorship W2053800551A5063905419 @default.
- W2053800551 hasAuthorship W2053800551A5091421622 @default.
- W2053800551 hasBestOaLocation W20538005511 @default.