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- W2053823786 abstract "HiLo microscopy is a recently developed technique that provides both optical sectioning and fast imaging with a simple implementation and at a very low cost. The methodology combines widefield and speckled illumination images to obtain one optically sectioned image. Hence, the characteristics of such speckle illumination ultimately determine the quality of HiLo images and the overall performance of the method. In this work, we study how speckle contrast influence local variations of fluorescence intensity and brightness profiles of thick samples. We present this article as a guide to adjust the parameters of the system for optimizing the capabilities of this novel technology." @default.
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- W2053823786 date "2011-07-13" @default.
- W2053823786 modified "2023-10-03" @default.
- W2053823786 title "Analyzing speckle contrast for HiLo microscopy optimization" @default.
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- W2053823786 doi "https://doi.org/10.1364/oe.19.014508" @default.
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