Matches in SemOpenAlex for { <https://semopenalex.org/work/W2053846917> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2053846917 endingPage "149" @default.
- W2053846917 startingPage "146" @default.
- W2053846917 abstract "Of more than 2 million segmental bone defects repaired annually with bone autografts and allografts, 15–40% fail. Improving healing rates may be approached with tissue engineering and use of periosteum overlying an allograft. The present study documents gene expression in human periosteum–allograft constructs compared to allografts alone. Strips of human cadaveric periosteum (26 years, f, distal femur) were sutured about sterilized human femoral cortical strut bone allograft (54 years, m) segments. After construct incubation (M199 supplemented medium) for 8 d, constructs and allografts alone were implanted in nude mice. At 10 and 20 weeks, constructs (N = 4, each group) and allografts (N = 2, each group) were retrieved and placed in RNAlater for quantitative PCR to determine expression of human- and murine-specific genes relevant to remodeling. Specimens were frozen-ground to powders and RNA was extracted, purified, reverse-transcribed, and amplified. Ribosomal protein (P0) was used to normalize sample quantities. Fold change plots were generated following statistical analyses comparing 20- to 10-week gene expression data. Allografts alone yielded no human-specific gene expression. Notable fold changes of human-specific alkaline phosphatase, bone sialoprotein, type I collagen, decorin, RANKL, RANK, cathepsin K, and osteocalcin in 20-week compared to 10-week specimens were found. Murine-specific expression of genes indicative of host mouse vascularization (RANK, type I collagen) was detected in both allograft alone and periosteum–allograft samples. Gene data confirm viable periosteum in constructs after 20 weeks. Relatively higher fold-change values of RANK, RANKL and cathepsin K indicate activities of osteoclast precursors, osteoclasts and osteoblasts involved in allograft remodeling during implantation. All additional genes of interest indicate osteoblast activity in new bone matrix formation. Gene data are directly correlated with previous and present histology work. The results of this study suggest that further investigations could help to establish whether autologous periosteum–allograft constructs could be used for the repair of bone defects." @default.
- W2053846917 created "2016-06-24" @default.
- W2053846917 creator A5021408163 @default.
- W2053846917 creator A5022170827 @default.
- W2053846917 creator A5035328125 @default.
- W2053846917 creator A5066049851 @default.
- W2053846917 creator A5066597221 @default.
- W2053846917 creator A5069021535 @default.
- W2053846917 date "2014-08-15" @default.
- W2053846917 modified "2023-10-18" @default.
- W2053846917 title "The character of gene expression of human periosteum used to form new tissue in allograft bone" @default.
- W2053846917 cites W2017300493 @default.
- W2053846917 cites W203494167 @default.
- W2053846917 cites W2038730751 @default.
- W2053846917 cites W2067932339 @default.
- W2053846917 cites W2106889572 @default.
- W2053846917 cites W2132439998 @default.
- W2053846917 cites W2156878231 @default.
- W2053846917 cites W2157848843 @default.
- W2053846917 cites W2163109093 @default.
- W2053846917 doi "https://doi.org/10.3109/03008207.2014.923851" @default.
- W2053846917 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25158200" @default.
- W2053846917 hasPublicationYear "2014" @default.
- W2053846917 type Work @default.
- W2053846917 sameAs 2053846917 @default.
- W2053846917 citedByCount "9" @default.
- W2053846917 countsByYear W20538469172015 @default.
- W2053846917 countsByYear W20538469172016 @default.
- W2053846917 countsByYear W20538469172017 @default.
- W2053846917 countsByYear W20538469172019 @default.
- W2053846917 countsByYear W20538469172020 @default.
- W2053846917 countsByYear W20538469172021 @default.
- W2053846917 countsByYear W20538469172022 @default.
- W2053846917 countsByYear W20538469172023 @default.
- W2053846917 crossrefType "journal-article" @default.
- W2053846917 hasAuthorship W2053846917A5021408163 @default.
- W2053846917 hasAuthorship W2053846917A5022170827 @default.
- W2053846917 hasAuthorship W2053846917A5035328125 @default.
- W2053846917 hasAuthorship W2053846917A5066049851 @default.
- W2053846917 hasAuthorship W2053846917A5066597221 @default.
- W2053846917 hasAuthorship W2053846917A5069021535 @default.
- W2053846917 hasConcept C104317684 @default.
- W2053846917 hasConcept C105702510 @default.
- W2053846917 hasConcept C131075544 @default.
- W2053846917 hasConcept C142724271 @default.
- W2053846917 hasConcept C150194340 @default.
- W2053846917 hasConcept C153911025 @default.
- W2053846917 hasConcept C160160445 @default.
- W2053846917 hasConcept C167844969 @default.
- W2053846917 hasConcept C181199279 @default.
- W2053846917 hasConcept C2776432469 @default.
- W2053846917 hasConcept C2777674748 @default.
- W2053846917 hasConcept C2779428903 @default.
- W2053846917 hasConcept C55493867 @default.
- W2053846917 hasConcept C71924100 @default.
- W2053846917 hasConcept C86803240 @default.
- W2053846917 hasConcept C88045685 @default.
- W2053846917 hasConceptScore W2053846917C104317684 @default.
- W2053846917 hasConceptScore W2053846917C105702510 @default.
- W2053846917 hasConceptScore W2053846917C131075544 @default.
- W2053846917 hasConceptScore W2053846917C142724271 @default.
- W2053846917 hasConceptScore W2053846917C150194340 @default.
- W2053846917 hasConceptScore W2053846917C153911025 @default.
- W2053846917 hasConceptScore W2053846917C160160445 @default.
- W2053846917 hasConceptScore W2053846917C167844969 @default.
- W2053846917 hasConceptScore W2053846917C181199279 @default.
- W2053846917 hasConceptScore W2053846917C2776432469 @default.
- W2053846917 hasConceptScore W2053846917C2777674748 @default.
- W2053846917 hasConceptScore W2053846917C2779428903 @default.
- W2053846917 hasConceptScore W2053846917C55493867 @default.
- W2053846917 hasConceptScore W2053846917C71924100 @default.
- W2053846917 hasConceptScore W2053846917C86803240 @default.
- W2053846917 hasConceptScore W2053846917C88045685 @default.
- W2053846917 hasIssue "sup1" @default.
- W2053846917 hasLocation W20538469171 @default.
- W2053846917 hasLocation W20538469172 @default.
- W2053846917 hasOpenAccess W2053846917 @default.
- W2053846917 hasPrimaryLocation W20538469171 @default.
- W2053846917 hasRelatedWork W1568198266 @default.
- W2053846917 hasRelatedWork W1981582835 @default.
- W2053846917 hasRelatedWork W1984617699 @default.
- W2053846917 hasRelatedWork W2028553359 @default.
- W2053846917 hasRelatedWork W2068520348 @default.
- W2053846917 hasRelatedWork W2109782233 @default.
- W2053846917 hasRelatedWork W2119219558 @default.
- W2053846917 hasRelatedWork W2136335883 @default.
- W2053846917 hasRelatedWork W2313637060 @default.
- W2053846917 hasRelatedWork W2522438288 @default.
- W2053846917 hasVolume "55" @default.
- W2053846917 isParatext "false" @default.
- W2053846917 isRetracted "false" @default.
- W2053846917 magId "2053846917" @default.
- W2053846917 workType "article" @default.