FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2053896453> ?p ?o ?g. }

• W2053896453 endingPage "41" @default.
• W2053896453 startingPage "29" @default.
• W2053896453 abstract "The serotonin transporter (SERT) is regulated by various signaling mechanisms that may operate to maintain appropriate levels of synaptic serotonin (5-HT). We demonstrate that one of the mitogen-activated protein kinases (MAPKs), p38 MAPK, regulates SERT. Treatment of rat midbrain synaptosomes with p38 MAPK-specific inhibitors, PD169316 [4-(4-fluorophenyl)-2-(4-nitrophenyl)-5-(4-pyridyl)-1H-imidazole] or SB203580 [4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-1H-imidazole], reduced 5-HT uptake. An additive SERT inhibition by PD169316 and β-phorbol 12-myristate 13-acetate (β-PMA) indicated the involvement of a protein kinase C (PKC)-independent MAPK pathway. Kinetic studies indicated a significant decrease in the transport capacity ( V max ) after PD169316 treatment of synaptosomes. Biotinylation studies showed reduced SERT proteins in the plasma membrane of synaptosomes after p38 MAPK inhibition and PKC activation. Phosphorylation studies using synaptosomes revealed decreased SERT phosphorylation by PD169316 but increased phosphorylation by β-PMA. d -Amphetamine enhanced SERT basal phosphorylation and PD169316 blocked this effect. SERT interaction with protein phosphatase 2A catalytic subunit and syntaxin 1A decreased after PD169316 or β-PMA treatment of synaptosomes. In synaptosomes, PKC activation but not p38 MAPK inhibition resulted in SERT redistribution from cholesterolrich lipid raft fractions to nonlipid raft fractions. The presence of phospho-p38 MAPK in synaptosomes and human embryonic kidney 293 (HEK-293) cells suggested the presence of constitutively active p38 MAPK in these preparations. Cotransfection of HEK-293 cells with SERT and a constitutively active form of MAP kinase kinase 3b(E) [MKK3b(E)] increased 5-HT transport, and RNA interference targeted to p38 MAPK inhibited 5-HT uptake, confirming the involvement of active p38 MAPK in SERT expression. Although PD169316 inhibited SERT insertion to the plasma membrane, β-PMA increased SERT internalization in HEK-293 cells. Together, these results indicate a distinct role of p38 MAPK in SERT regulation." @default.
• W2053896453 created "2016-06-24" @default.
• W2053896453 creator A5000509037 @default.
• W2053896453 creator A5063658897 @default.
• W2053896453 creator A5083691375 @default.
• W2053896453 creator A5089787351 @default.
• W2053896453 date "2005-01-05" @default.
• W2053896453 modified "2023-09-30" @default.
• W2053896453 title "A Role for p38 Mitogen-Activated Protein Kinase in the Regulation of the Serotonin Transporter: Evidence for Distinct Cellular Mechanisms Involved in Transporter Surface Expression" @default.
• W2053896453 cites W1028523628 @default.
• W2053896453 cites W1570313229 @default.
• W2053896453 cites W1599164179 @default.
• W2053896453 cites W1604642620 @default.
• W2053896453 cites W1609863923 @default.
• W2053896453 cites W1778282426 @default.
• W2053896453 cites W1828538530 @default.
• W2053896453 cites W1848879777 @default.
• W2053896453 cites W1911240071 @default.
• W2053896453 cites W1931583840 @default.
• W2053896453 cites W1964672651 @default.
• W2053896453 cites W1988715014 @default.
• W2053896453 cites W1988800128 @default.
• W2053896453 cites W1991335283 @default.
• W2053896453 cites W1994637967 @default.
• W2053896453 cites W1995283839 @default.
• W2053896453 cites W2000904449 @default.
• W2053896453 cites W2015068634 @default.
• W2053896453 cites W2015802741 @default.
• W2053896453 cites W2016532705 @default.
• W2053896453 cites W2020188308 @default.
• W2053896453 cites W2024790646 @default.
• W2053896453 cites W2026307239 @default.
• W2053896453 cites W2026403124 @default.
• W2053896453 cites W2030373257 @default.
• W2053896453 cites W2034703968 @default.
• W2053896453 cites W2037406484 @default.
• W2053896453 cites W2052252808 @default.
• W2053896453 cites W2054927476 @default.
• W2053896453 cites W2055821738 @default.
• W2053896453 cites W2056033864 @default.
• W2053896453 cites W2065192682 @default.
• W2053896453 cites W2066138070 @default.
• W2053896453 cites W2067827045 @default.
• W2053896453 cites W2068630247 @default.
• W2053896453 cites W2079040386 @default.
• W2053896453 cites W2081694946 @default.
• W2053896453 cites W2082450845 @default.
• W2053896453 cites W2085357088 @default.
• W2053896453 cites W2091649030 @default.
• W2053896453 cites W2092296126 @default.
• W2053896453 cites W2092395511 @default.
• W2053896453 cites W2093414544 @default.
• W2053896453 cites W2095152292 @default.
• W2053896453 cites W2097032963 @default.
• W2053896453 cites W2100212868 @default.
• W2053896453 cites W2103745296 @default.
• W2053896453 cites W2103926307 @default.
• W2053896453 cites W2121936687 @default.
• W2053896453 cites W2128763007 @default.
• W2053896453 cites W2146092354 @default.
• W2053896453 cites W2149863291 @default.
• W2053896453 cites W2153636452 @default.
• W2053896453 cites W2155993884 @default.
• W2053896453 cites W2160253297 @default.
• W2053896453 cites W2164967571 @default.
• W2053896453 cites W2262635949 @default.
• W2053896453 cites W4237354829 @default.
• W2053896453 cites W74063644 @default.
• W2053896453 doi "https://doi.org/10.1523/jneurosci.3754-04.2005" @default.
• W2053896453 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6725216" @default.
• W2053896453 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15634764" @default.
• W2053896453 hasPublicationYear "2005" @default.
• W2053896453 type Work @default.
• W2053896453 sameAs 2053896453 @default.
• W2053896453 citedByCount "196" @default.
• W2053896453 countsByYear W20538964532012 @default.
• W2053896453 countsByYear W20538964532013 @default.
• W2053896453 countsByYear W20538964532014 @default.
• W2053896453 countsByYear W20538964532015 @default.
• W2053896453 countsByYear W20538964532016 @default.
• W2053896453 countsByYear W20538964532017 @default.
• W2053896453 countsByYear W20538964532018 @default.
• W2053896453 countsByYear W20538964532019 @default.
• W2053896453 countsByYear W20538964532020 @default.
• W2053896453 countsByYear W20538964532021 @default.
• W2053896453 countsByYear W20538964532022 @default.
• W2053896453 countsByYear W20538964532023 @default.
• W2053896453 crossrefType "journal-article" @default.
• W2053896453 hasAuthorship W2053896453A5000509037 @default.
• W2053896453 hasAuthorship W2053896453A5063658897 @default.
• W2053896453 hasAuthorship W2053896453A5083691375 @default.
• W2053896453 hasAuthorship W2053896453A5089787351 @default.
• W2053896453 hasBestOaLocation W20538964531 @default.
• W2053896453 hasConcept C11960822 @default.
• W2053896453 hasConcept C153911025 @default.
• W2053896453 hasConcept C170493617 @default.
• W2053896453 hasConcept C184235292 @default.
• W2053896453 hasConcept C185592680 @default.

• next