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- W2053919152 abstract "Simultaneous biodegradation of hexachlorocyclohexane (HCH) and organophosphates (OPs) by a recombinant Stenotrophomonas sp. was studied in the study. The broad-host-range plasmid pVGAB, harboring enhanced green fluorescent protein gene (egfp) and dehalogenase genes (linA and linB), was constructed and transformed into the OP-degrading strain Stenotrophomonas YC-1 to get the recombinant strain YC-H. Over-expression of dehalogenase (LinA and LinB) and enhanced green fluorescent protein (EGFP) was obtained in YC-H by determining their enzymatic activities and fluorescence intensity. YC-H was capable of rapidly and simultaneously degrading 10 mg/l γ-HCH and 100 mg/l methyl parathion (MP) determined by GC–ECD analysis. A bioremediation assay with YC-H inoculated into fumigated and nonfumigated soil showed that both 10 mg/kg γ-HCH and 100 mg/kg MP could be completely degraded within 32 days. The novel EGFP-marked bacterium could be potentially applied in the field-scale decontamination of HCH and OPs residues in the environment." @default.
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- W2053919152 date "2009-07-01" @default.
- W2053919152 modified "2023-10-18" @default.
- W2053919152 title "Development of an autofluorescent organophosphates-degrading Stenotrophomonas sp. with dehalogenase activity for the biodegradation of hexachlorocyclohexane (HCH)" @default.
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- W2053919152 doi "https://doi.org/10.1016/j.biortech.2009.02.008" @default.
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