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• W2053980147 abstract "Summary Although huge amounts of high‐throughput sequencing ( HTS ) data are available, limited systematic analyses have been performed by integrating these valuable resources. Based on small RNA (s RNA ), RNA and degradome HTS data, the s RNA s specifically expressed at vegetative and reproductive stages were identified separately in rice. Two distinct groups of s RNA HTS data, which were prepared during the vegetative and the reproductive stages, were utilized to extract stage‐specific s RNA s. Degradome sequencing data were employed for s RNA target validation. RNA sequencing data were used to construct expression‐based, s RNA ‐mediated networks. As a result, 26 micro RNA s and 413 s RNA s were specifically expressed at the vegetative stage, and 79 micro RNA s and 539 s RNA s were specifically expressed at the reproductive stage. In addition to the micro RNA s, numerous stage‐specific s RNA s enriched in ARGONAUTE 1 showed great potential to perform cleavage‐based repression on the targets. Several stage‐specific s RNA s were indicated to result from the wobble effect of D icer‐like 1‐mediated processing of micro RNA precursors. The expression patterns of the s RNA targets, and the stage‐specific cleavage signals strongly indicated the reliability of the constructed networks. A set of rice stage‐specific s RNA s along with the regulatory cascades, which have great potential in regulating specific developmental stages, were provided for further investigation." @default.
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• W2053980147 date "2012-11-05" @default.
• W2053980147 modified "2023-10-16" @default.
• W2053980147 title "Construction of gene regulatory networks mediated by vegetative and reproductive stage‐specific small <scp>RNA</scp> s in rice ( <i> <scp>O</scp> ryza sativa </i> )" @default.
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• W2053980147 doi "https://doi.org/10.1111/nph.12018" @default.
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