FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2053982014> ?p ?o ?g. }

• W2053982014 endingPage "7" @default.
• W2053982014 startingPage "1" @default.
• W2053982014 abstract "The receptor subtypes, which mediate nicotine-induced excitation of dopaminergic neurons in the substantia nigra, were investigated by whole-cell patch clamp studies and single-cell RT-PCR using acutely dissociated nigral neurons. Three types of current were observed when acetylcholine (1 mM) was applied to the neurons in the presence of atropine (1 μM) by the U-tube system, which allowed the rapid application of drugs. In 50% of neurons examined, acetylcholine (1 mM) plus atropine (1 μM) evoked a current with a rapidly desensitizing decay phase (designated as type Ia current). In 14% of neurons tested, the current induced by acetylcholine plus atropine had a decay phase with slow desensitization (designated as type II current). The third type of response, which had both characteristics of type Ia and II currents, was evoked in 36% of neurons tested (designated as type Ib currents). Nicotine (1 mM) also induced three types of inward currents which were similar to those induced by acetylcholine (1 mM) plus atropine (1 μM). In all three types of current, nicotine (0.1 μM–1 mM)-evoked inward currents were dose-dependent. Type Ia and II currents were inhibited by methyllycaconitine (MLA, 0.01 μM), a selective nicotinic α7 receptor antagonist, and dihydro-β-erythroidine (DHβE, 0.1 μM), an antagonist for α4β2 receptor, respectively. In type Ib currents, a fast-decaying phase was inhibited by MLA (0.01 μM), while a slow-decaying phase was blocked by DHβE (0.1μM). After recording the type Ib current, single-cell RT-PCR analysis was performed using aspirated cytoplasm as total RNA templates. The results revealed that mRNAs for α7 nicotinic receptor subunit and tyrosine hydroxylase were detected in the same single neuron tested, which confirms the existence of α7-type nicotinic acetylcholine receptor in dopaminergic neurons of this area. These results suggest that nicotine directly acts on postsynaptic α7- and α4β2-type nicotinic acetylcholine receptors and induces inward current, which result in the excitation of dopaminergic neurons in the substantia nigra." @default.
• W2053982014 created "2016-06-24" @default.
• W2053982014 creator A5017413368 @default.
• W2053982014 creator A5019881008 @default.
• W2053982014 creator A5031749767 @default.
• W2053982014 creator A5037637753 @default.
• W2053982014 creator A5057339710 @default.
• W2053982014 creator A5070273099 @default.
• W2053982014 creator A5072910282 @default.
• W2053982014 date "2004-10-01" @default.
• W2053982014 modified "2023-10-11" @default.
• W2053982014 title "Involvement of α7- and α4β2-type postsynaptic nicotinic acetylcholine receptors in nicotine-induced excitation of dopaminergic neurons in the substantia nigra: a patch clamp and single-cell PCR study using acutely dissociated nigral neurons" @default.
• W2053982014 cites W1608212407 @default.
• W2053982014 cites W1963492897 @default.
• W2053982014 cites W1970440438 @default.
• W2053982014 cites W1973059761 @default.
• W2053982014 cites W1986122184 @default.
• W2053982014 cites W1986469936 @default.
• W2053982014 cites W1997150127 @default.
• W2053982014 cites W2003415102 @default.
• W2053982014 cites W2009667219 @default.
• W2053982014 cites W2022616614 @default.
• W2053982014 cites W2024970998 @default.
• W2053982014 cites W2046843095 @default.
• W2053982014 cites W2057780012 @default.
• W2053982014 cites W2122921281 @default.
• W2053982014 cites W4322696256 @default.
• W2053982014 doi "https://doi.org/10.1016/j.molbrainres.2004.06.040" @default.
• W2053982014 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15469877" @default.
• W2053982014 hasPublicationYear "2004" @default.
• W2053982014 type Work @default.
• W2053982014 sameAs 2053982014 @default.
• W2053982014 citedByCount "36" @default.
• W2053982014 countsByYear W20539820142014 @default.
• W2053982014 countsByYear W20539820142015 @default.
• W2053982014 countsByYear W20539820142016 @default.
• W2053982014 countsByYear W20539820142017 @default.
• W2053982014 countsByYear W20539820142018 @default.
• W2053982014 countsByYear W20539820142019 @default.
• W2053982014 countsByYear W20539820142020 @default.
• W2053982014 countsByYear W20539820142022 @default.
• W2053982014 crossrefType "journal-article" @default.
• W2053982014 hasAuthorship W2053982014A5017413368 @default.
• W2053982014 hasAuthorship W2053982014A5019881008 @default.
• W2053982014 hasAuthorship W2053982014A5031749767 @default.
• W2053982014 hasAuthorship W2053982014A5037637753 @default.
• W2053982014 hasAuthorship W2053982014A5057339710 @default.
• W2053982014 hasAuthorship W2053982014A5070273099 @default.
• W2053982014 hasAuthorship W2053982014A5072910282 @default.
• W2053982014 hasConcept C126322002 @default.
• W2053982014 hasConcept C134018914 @default.
• W2053982014 hasConcept C137183658 @default.
• W2053982014 hasConcept C147944092 @default.
• W2053982014 hasConcept C169760540 @default.
• W2053982014 hasConcept C170493617 @default.
• W2053982014 hasConcept C185263204 @default.
• W2053982014 hasConcept C188987157 @default.
• W2053982014 hasConcept C2775859210 @default.
• W2053982014 hasConcept C2775910092 @default.
• W2053982014 hasConcept C2779547902 @default.
• W2053982014 hasConcept C2779570518 @default.
• W2053982014 hasConcept C2780374058 @default.
• W2053982014 hasConcept C2780938664 @default.
• W2053982014 hasConcept C513476851 @default.
• W2053982014 hasConcept C71924100 @default.
• W2053982014 hasConcept C80161118 @default.
• W2053982014 hasConcept C86803240 @default.
• W2053982014 hasConceptScore W2053982014C126322002 @default.
• W2053982014 hasConceptScore W2053982014C134018914 @default.
• W2053982014 hasConceptScore W2053982014C137183658 @default.
• W2053982014 hasConceptScore W2053982014C147944092 @default.
• W2053982014 hasConceptScore W2053982014C169760540 @default.
• W2053982014 hasConceptScore W2053982014C170493617 @default.
• W2053982014 hasConceptScore W2053982014C185263204 @default.
• W2053982014 hasConceptScore W2053982014C188987157 @default.
• W2053982014 hasConceptScore W2053982014C2775859210 @default.
• W2053982014 hasConceptScore W2053982014C2775910092 @default.
• W2053982014 hasConceptScore W2053982014C2779547902 @default.
• W2053982014 hasConceptScore W2053982014C2779570518 @default.
• W2053982014 hasConceptScore W2053982014C2780374058 @default.
• W2053982014 hasConceptScore W2053982014C2780938664 @default.
• W2053982014 hasConceptScore W2053982014C513476851 @default.
• W2053982014 hasConceptScore W2053982014C71924100 @default.
• W2053982014 hasConceptScore W2053982014C80161118 @default.
• W2053982014 hasConceptScore W2053982014C86803240 @default.
• W2053982014 hasIssue "1-2" @default.
• W2053982014 hasLocation W20539820141 @default.
• W2053982014 hasLocation W20539820142 @default.
• W2053982014 hasOpenAccess W2053982014 @default.
• W2053982014 hasPrimaryLocation W20539820141 @default.
• W2053982014 hasRelatedWork W1798538792 @default.
• W2053982014 hasRelatedWork W1974783664 @default.
• W2053982014 hasRelatedWork W2005226742 @default.
• W2053982014 hasRelatedWork W2024552379 @default.
• W2053982014 hasRelatedWork W2044186126 @default.
• W2053982014 hasRelatedWork W2056652910 @default.
• W2053982014 hasRelatedWork W2061901670 @default.
• W2053982014 hasRelatedWork W2198319348 @default.

• next