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- W2053985915 abstract "Cultures of rat cerebellum and spinal cord were used to visualize binding sites for [3Hγaminobutyrate, [3Hmuscimol, [3Hbicuculline methiodide and [3Hflunitrazepam by autoradiography. In cerebellar cultures, many large neurons (presumably Purkinje cells) and interneurons were labelled. In spinal cord cultures, these compounds were mainly bound to small and medium-sized neurons, whereas the majority of large neurons were unlabelled. No binding sites for these radioligands were found on glial cells. Binding of [3Hγ-aminobutyrate, [3Hmuscimol and [3Hbicuculline methiodide was markedly reduced or inhibited by adding unlabelled γ-aminobutyrate, muscimol and bicuculline (10−3m) respectively to the incubation medium. Addition of a thienobenzazepine markedly reduced binding with [3Hflunitrazepam. It is concluded that tissue cultures are an excellent tool to visualize the cellular localization of binding sites for neurotransmitters and drugs using autoradiography." @default.
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- W2053985915 title "Autoradiographic localization of binding sites for [3Hγ-aminobutyrate, [3Hmuscimol, ( + )[3Hbicuculline methiodide and [3H flunitrazepam in cultures of rat cerebellum and spinal cord" @default.
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- W2053985915 doi "https://doi.org/10.1016/0306-4522(80)90029-9" @default.
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