FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2054010758> ?p ?o ?g. }

• W2054010758 endingPage "11" @default.
• W2054010758 startingPage "1" @default.
• W2054010758 abstract "G protein-coupled receptors (GPCRs) are associated with a great variety of biological activities. Yeasts are often utilized as a host for heterologous GPCR assay. We engineered the intense reporter plasmids for fission yeast to produce green fluorescent protein (GFP) through its endogenous GPCR pathway. As a control region of GFP expression on the reporter plasmid, we focused on seven endogenous genes specifically activated through the pathway. When upstream regions of these genes were used as an inducible promoter in combination with LPI terminator, the mam2 upstream region produced GFP most rapidly and intensely despite the high background. Subsequently, LPI terminator was replaced with the corresponding downstream regions. The SPBC4.01 downstream region enhanced the response with the low background. Furthermore, combining SPBC4.01 downstream region with the sxa2 upstream region, the signal to noise ratio was obviously better than those of other regions. We also evaluated the time- and dose-dependent GFP productions of the strains transformed with the reporter plasmids. Finally, we exhibited a model of simplified GPCR assay with the reporter plasmid by expressing endogenous GPCR under the control of the foreign promoter." @default.
• W2054010758 created "2016-06-24" @default.
• W2054010758 creator A5001125537 @default.
• W2054010758 creator A5052329624 @default.
• W2054010758 date "2012-01-01" @default.
• W2054010758 modified "2023-10-05" @default.
• W2054010758 title "The Reporter System for GPCR Assay with the Fission YeastSchizosaccharomyces pombe" @default.
• W2054010758 cites W1486604407 @default.
• W2054010758 cites W1547901668 @default.
• W2054010758 cites W1592607632 @default.
• W2054010758 cites W1715927468 @default.
• W2054010758 cites W1904970865 @default.
• W2054010758 cites W1977982633 @default.
• W2054010758 cites W1979708251 @default.
• W2054010758 cites W1980585800 @default.
• W2054010758 cites W1985738852 @default.
• W2054010758 cites W1992981247 @default.
• W2054010758 cites W1994086256 @default.
• W2054010758 cites W1996612953 @default.
• W2054010758 cites W2011369177 @default.
• W2054010758 cites W2011558569 @default.
• W2054010758 cites W2013208933 @default.
• W2054010758 cites W2015267380 @default.
• W2054010758 cites W2032233401 @default.
• W2054010758 cites W2049222500 @default.
• W2054010758 cites W2055684843 @default.
• W2054010758 cites W2058582467 @default.
• W2054010758 cites W2067403630 @default.
• W2054010758 cites W2069229484 @default.
• W2054010758 cites W2071497657 @default.
• W2054010758 cites W2071597689 @default.
• W2054010758 cites W2084467989 @default.
• W2054010758 cites W2092436603 @default.
• W2054010758 cites W2097186487 @default.
• W2054010758 cites W2107258432 @default.
• W2054010758 cites W2131419026 @default.
• W2054010758 cites W2131977749 @default.
• W2054010758 cites W2132899947 @default.
• W2054010758 cites W2138657668 @default.
• W2054010758 cites W2141976844 @default.
• W2054010758 cites W2147436067 @default.
• W2054010758 doi "https://doi.org/10.6064/2012/674256" @default.
• W2054010758 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3820654" @default.
• W2054010758 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24278726" @default.
• W2054010758 hasPublicationYear "2012" @default.
• W2054010758 type Work @default.
• W2054010758 sameAs 2054010758 @default.
• W2054010758 citedByCount "1" @default.
• W2054010758 countsByYear W20540107582019 @default.
• W2054010758 crossrefType "journal-article" @default.
• W2054010758 hasAuthorship W2054010758A5001125537 @default.
• W2054010758 hasAuthorship W2054010758A5052329624 @default.
• W2054010758 hasBestOaLocation W20540107581 @default.
• W2054010758 hasConcept C104317684 @default.
• W2054010758 hasConcept C116403925 @default.
• W2054010758 hasConcept C121332964 @default.
• W2054010758 hasConcept C1276947 @default.
• W2054010758 hasConcept C135285700 @default.
• W2054010758 hasConcept C142613039 @default.
• W2054010758 hasConcept C150194340 @default.
• W2054010758 hasConcept C153911025 @default.
• W2054010758 hasConcept C161733203 @default.
• W2054010758 hasConcept C16613235 @default.
• W2054010758 hasConcept C178046730 @default.
• W2054010758 hasConcept C22744801 @default.
• W2054010758 hasConcept C2780115692 @default.
• W2054010758 hasConcept C54355233 @default.
• W2054010758 hasConcept C55493867 @default.
• W2054010758 hasConcept C62478195 @default.
• W2054010758 hasConcept C86803240 @default.
• W2054010758 hasConcept C95444343 @default.
• W2054010758 hasConceptScore W2054010758C104317684 @default.
• W2054010758 hasConceptScore W2054010758C116403925 @default.
• W2054010758 hasConceptScore W2054010758C121332964 @default.
• W2054010758 hasConceptScore W2054010758C1276947 @default.
• W2054010758 hasConceptScore W2054010758C135285700 @default.
• W2054010758 hasConceptScore W2054010758C142613039 @default.
• W2054010758 hasConceptScore W2054010758C150194340 @default.
• W2054010758 hasConceptScore W2054010758C153911025 @default.
• W2054010758 hasConceptScore W2054010758C161733203 @default.
• W2054010758 hasConceptScore W2054010758C16613235 @default.
• W2054010758 hasConceptScore W2054010758C178046730 @default.
• W2054010758 hasConceptScore W2054010758C22744801 @default.
• W2054010758 hasConceptScore W2054010758C2780115692 @default.
• W2054010758 hasConceptScore W2054010758C54355233 @default.
• W2054010758 hasConceptScore W2054010758C55493867 @default.
• W2054010758 hasConceptScore W2054010758C62478195 @default.
• W2054010758 hasConceptScore W2054010758C86803240 @default.
• W2054010758 hasConceptScore W2054010758C95444343 @default.
• W2054010758 hasFunder F4320334764 @default.
• W2054010758 hasLocation W20540107581 @default.
• W2054010758 hasLocation W20540107582 @default.
• W2054010758 hasLocation W20540107583 @default.
• W2054010758 hasLocation W20540107584 @default.
• W2054010758 hasLocation W20540107585 @default.
• W2054010758 hasOpenAccess W2054010758 @default.
• W2054010758 hasPrimaryLocation W20540107581 @default.
• W2054010758 hasRelatedWork W2010668679 @default.

• next