Matches in SemOpenAlex for { <https://semopenalex.org/work/W2054018509> ?p ?o ?g. }
- W2054018509 endingPage "1536" @default.
- W2054018509 startingPage "1527" @default.
- W2054018509 abstract "Diflavin reductases are bidomain electron transfer proteins in which structural reorientation is necessary to account for the various intramolecular and intermolecular electron transfer steps. Using small-angle x-ray scattering and nuclear magnetic resonance data, we describe the conformational free-energy landscape of the NADPH-cytochrome P450 reductase (CPR), a typical bidomain redox enzyme composed of two covalently-bound flavin domains, under various experimental conditions. The CPR enzyme exists in a salt- and pH-dependent rapid equilibrium between a previously described rigid, locked state and a newly characterized, highly flexible, unlocked state. We further establish that maximal electron flux through CPR is conditioned by adjustable stability of the locked-state domain interface under resting conditions. This is rationalized by a kinetic scheme coupling rapid conformational sampling and slow chemical reaction rates. Regulated domain interface stability associated with fast stochastic domain contacts during the catalytic cycle thus provides, to our knowledge, a new paradigm for improving our understanding of multidomain enzyme function." @default.
- W2054018509 created "2016-06-24" @default.
- W2054018509 creator A5028259435 @default.
- W2054018509 creator A5028382987 @default.
- W2054018509 creator A5030805030 @default.
- W2054018509 creator A5044887967 @default.
- W2054018509 creator A5055365455 @default.
- W2054018509 creator A5062503534 @default.
- W2054018509 creator A5078539437 @default.
- W2054018509 creator A5081130053 @default.
- W2054018509 date "2015-03-01" @default.
- W2054018509 modified "2023-10-12" @default.
- W2054018509 title "A Well-Balanced Preexisting Equilibrium Governs Electron Flux Efficiency of a Multidomain Diflavin Reductase" @default.
- W2054018509 cites W1488793801 @default.
- W2054018509 cites W1586605199 @default.
- W2054018509 cites W1693242556 @default.
- W2054018509 cites W1971317954 @default.
- W2054018509 cites W1974302220 @default.
- W2054018509 cites W1986080936 @default.
- W2054018509 cites W2001804573 @default.
- W2054018509 cites W2003105919 @default.
- W2054018509 cites W2003672482 @default.
- W2054018509 cites W2004713885 @default.
- W2054018509 cites W2006465601 @default.
- W2054018509 cites W2012580167 @default.
- W2054018509 cites W2021296090 @default.
- W2054018509 cites W2029903300 @default.
- W2054018509 cites W2041300634 @default.
- W2054018509 cites W2044250235 @default.
- W2054018509 cites W2046700770 @default.
- W2054018509 cites W2050906162 @default.
- W2054018509 cites W2053335879 @default.
- W2054018509 cites W2062715262 @default.
- W2054018509 cites W2064884941 @default.
- W2054018509 cites W2064918930 @default.
- W2054018509 cites W2068143879 @default.
- W2054018509 cites W2069602336 @default.
- W2054018509 cites W2072654958 @default.
- W2054018509 cites W2073142322 @default.
- W2054018509 cites W2074570464 @default.
- W2054018509 cites W2078109757 @default.
- W2054018509 cites W2079676299 @default.
- W2054018509 cites W2079732359 @default.
- W2054018509 cites W2092943003 @default.
- W2054018509 cites W2094484494 @default.
- W2054018509 cites W2096587470 @default.
- W2054018509 cites W2140246461 @default.
- W2054018509 cites W2142334255 @default.
- W2054018509 cites W2145160029 @default.
- W2054018509 cites W2162942780 @default.
- W2054018509 cites W2163488599 @default.
- W2054018509 cites W2317456426 @default.
- W2054018509 cites W2325252308 @default.
- W2054018509 cites W2331602804 @default.
- W2054018509 doi "https://doi.org/10.1016/j.bpj.2015.01.032" @default.
- W2054018509 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4375552" @default.
- W2054018509 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25809265" @default.
- W2054018509 hasPublicationYear "2015" @default.
- W2054018509 type Work @default.
- W2054018509 sameAs 2054018509 @default.
- W2054018509 citedByCount "30" @default.
- W2054018509 countsByYear W20540185092016 @default.
- W2054018509 countsByYear W20540185092017 @default.
- W2054018509 countsByYear W20540185092018 @default.
- W2054018509 countsByYear W20540185092019 @default.
- W2054018509 countsByYear W20540185092020 @default.
- W2054018509 countsByYear W20540185092021 @default.
- W2054018509 countsByYear W20540185092022 @default.
- W2054018509 countsByYear W20540185092023 @default.
- W2054018509 crossrefType "journal-article" @default.
- W2054018509 hasAuthorship W2054018509A5028259435 @default.
- W2054018509 hasAuthorship W2054018509A5028382987 @default.
- W2054018509 hasAuthorship W2054018509A5030805030 @default.
- W2054018509 hasAuthorship W2054018509A5044887967 @default.
- W2054018509 hasAuthorship W2054018509A5055365455 @default.
- W2054018509 hasAuthorship W2054018509A5062503534 @default.
- W2054018509 hasAuthorship W2054018509A5078539437 @default.
- W2054018509 hasAuthorship W2054018509A5081130053 @default.
- W2054018509 hasBestOaLocation W20540185091 @default.
- W2054018509 hasConcept C123669783 @default.
- W2054018509 hasConcept C159467904 @default.
- W2054018509 hasConcept C166950319 @default.
- W2054018509 hasConcept C178790620 @default.
- W2054018509 hasConcept C181199279 @default.
- W2054018509 hasConcept C185592680 @default.
- W2054018509 hasConcept C24840226 @default.
- W2054018509 hasConcept C32909587 @default.
- W2054018509 hasConcept C33093398 @default.
- W2054018509 hasConcept C55493867 @default.
- W2054018509 hasConcept C63338738 @default.
- W2054018509 hasConcept C71240020 @default.
- W2054018509 hasConcept C75079739 @default.
- W2054018509 hasConcept C75473681 @default.
- W2054018509 hasConceptScore W2054018509C123669783 @default.
- W2054018509 hasConceptScore W2054018509C159467904 @default.
- W2054018509 hasConceptScore W2054018509C166950319 @default.
- W2054018509 hasConceptScore W2054018509C178790620 @default.
- W2054018509 hasConceptScore W2054018509C181199279 @default.