FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2054029713> ?p ?o ?g. }

• W2054029713 abstract "Leishmaniasis is one of the world's most neglected diseases, largely affecting the poorest of the poor, mainly in developing countries. Over 350 million people are considered at risk of contracting leishmaniasis, and approximately 2 million new cases occur yearly(1). Leishmania donovani is the causative agent for visceral leishmaniasis (VL), the most fatal form of the disease. The choice of drugs available to treat leishmaniasis is limited (2);current treatments provide limited efficacy and many are toxic at therapeutic doses. In addition, most of the first line treatment drugs have already lost their utility due to increasing multiple drug resistance (3). The current pipeline of anti-leishmanial drugs is also severely depleted. Sustained efforts are needed to enrich a new anti-leishmanial drug discovery pipeline, and this endeavor relies on the availability of suitable in vitro screening models. In vitro promastigotes (4) and axenic amastigotes assays(5) are primarily used for anti-leishmanial drug screening however, may not be appropriate due to significant cellular, physiological, biochemical and molecular differences in comparison to intracellular amastigotes. Assays with macrophage-amastigotes models are considered closest to the pathophysiological conditions of leishmaniasis, and are therefore the most appropriate for in vitro screening. Differentiated, non-dividing human acute monocytic leukemia cells (THP1) (make an attractive) alternative to isolated primary macrophages and can be used for assaying anti-leishmanial activity of different compounds against intracellular amastigotes. Here, we present a parasite-rescue and transformation assay with differentiated THP1 cells infected in vitro with Leishmania donovani for screening pure compounds and natural products extracts and determining the efficacy against the intracellular Leishmania amastigotes. The assay involves the following steps: (1) differentiation of THP1 cells to non-dividing macrophages, (2) infection of macrophages with L. donovani metacyclic promastigotes, (3) treatment of infected cells with test drugs, (4) controlled lysis of infected macrophages, (5) release/rescue of amastigotes and (6) transformation of live amastigotes to promastigotes. The assay was optimized using detergent treatment for controlled lysis of Leishmania-infected THP1 cells to achieve almost complete rescue of viable intracellular amastigotes with minimal effect on their ability to transform to promastigotes. Different macrophage:promastigotes ratios were tested to achieve maximum infection. Quantification of the infection was performed through transformation of live, rescued Leishmania amastigotes to promastigotes and evaluation of their growth by an alamarBlue fluorometric assay in 96-well microplates. This assay is comparable to the currently-used microscopic, transgenic reporter gene and digital-image analysis assays. This assay is robust and measures only the live intracellular amastigotes compared to reporter gene and image analysis assays, which may not differentiate between live and dead amastigotes. Also, the assay has been validated with a current panel of anti-leishmanial drugs and has been successfully applied to large-scale screening of pure compounds and a library of natural products fractions (Tekwani et al. unpublished)." @default.
• W2054029713 created "2016-06-24" @default.
• W2054029713 creator A5014644608 @default.
• W2054029713 creator A5018778280 @default.
• W2054029713 creator A5087656284 @default.
• W2054029713 creator A5090532505 @default.
• W2054029713 date "2012-12-30" @default.
• W2054029713 modified "2023-10-14" @default.
• W2054029713 title "A Parasite Rescue and Transformation Assay for Antileishmanial Screening Against Intracellular <em>Leishmania donovani </em>Amastigotes in THP1 Human Acute Monocytic Leukemia Cell Line" @default.
• W2054029713 cites W126985493 @default.
• W2054029713 cites W1479952727 @default.
• W2054029713 cites W1501405435 @default.
• W2054029713 cites W1546173330 @default.
• W2054029713 cites W1564221315 @default.
• W2054029713 cites W1963542761 @default.
• W2054029713 cites W1997545346 @default.
• W2054029713 cites W2007552089 @default.
• W2054029713 cites W2016017024 @default.
• W2054029713 cites W2025078939 @default.
• W2054029713 cites W2039461307 @default.
• W2054029713 cites W2093642020 @default.
• W2054029713 cites W2100521145 @default.
• W2054029713 cites W2109803080 @default.
• W2054029713 cites W2113811423 @default.
• W2054029713 cites W2139456667 @default.
• W2054029713 cites W2949284957 @default.
• W2054029713 doi "https://doi.org/10.3791/4054" @default.
• W2054029713 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3577863" @default.
• W2054029713 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23299097" @default.
• W2054029713 hasPublicationYear "2012" @default.
• W2054029713 type Work @default.
• W2054029713 sameAs 2054029713 @default.
• W2054029713 citedByCount "70" @default.
• W2054029713 countsByYear W20540297132014 @default.
• W2054029713 countsByYear W20540297132015 @default.
• W2054029713 countsByYear W20540297132016 @default.
• W2054029713 countsByYear W20540297132017 @default.
• W2054029713 countsByYear W20540297132018 @default.
• W2054029713 countsByYear W20540297132019 @default.
• W2054029713 countsByYear W20540297132020 @default.
• W2054029713 countsByYear W20540297132021 @default.
• W2054029713 countsByYear W20540297132022 @default.
• W2054029713 countsByYear W20540297132023 @default.
• W2054029713 crossrefType "journal-article" @default.
• W2054029713 hasAuthorship W2054029713A5014644608 @default.
• W2054029713 hasAuthorship W2054029713A5018778280 @default.
• W2054029713 hasAuthorship W2054029713A5087656284 @default.
• W2054029713 hasAuthorship W2054029713A5090532505 @default.
• W2054029713 hasBestOaLocation W20540297131 @default.
• W2054029713 hasConcept C136764020 @default.
• W2054029713 hasConcept C153726182 @default.
• W2054029713 hasConcept C203014093 @default.
• W2054029713 hasConcept C2776555147 @default.
• W2054029713 hasConcept C2778689377 @default.
• W2054029713 hasConcept C2778702967 @default.
• W2054029713 hasConcept C2780599195 @default.
• W2054029713 hasConcept C2781092759 @default.
• W2054029713 hasConcept C2781333301 @default.
• W2054029713 hasConcept C41008148 @default.
• W2054029713 hasConcept C71928629 @default.
• W2054029713 hasConcept C86803240 @default.
• W2054029713 hasConcept C8891405 @default.
• W2054029713 hasConcept C98274493 @default.
• W2054029713 hasConcept C98319499 @default.
• W2054029713 hasConceptScore W2054029713C136764020 @default.
• W2054029713 hasConceptScore W2054029713C153726182 @default.
• W2054029713 hasConceptScore W2054029713C203014093 @default.
• W2054029713 hasConceptScore W2054029713C2776555147 @default.
• W2054029713 hasConceptScore W2054029713C2778689377 @default.
• W2054029713 hasConceptScore W2054029713C2778702967 @default.
• W2054029713 hasConceptScore W2054029713C2780599195 @default.
• W2054029713 hasConceptScore W2054029713C2781092759 @default.
• W2054029713 hasConceptScore W2054029713C2781333301 @default.
• W2054029713 hasConceptScore W2054029713C41008148 @default.
• W2054029713 hasConceptScore W2054029713C71928629 @default.
• W2054029713 hasConceptScore W2054029713C86803240 @default.
• W2054029713 hasConceptScore W2054029713C8891405 @default.
• W2054029713 hasConceptScore W2054029713C98274493 @default.
• W2054029713 hasConceptScore W2054029713C98319499 @default.
• W2054029713 hasIssue "70" @default.
• W2054029713 hasLocation W20540297131 @default.
• W2054029713 hasLocation W20540297132 @default.
• W2054029713 hasLocation W20540297133 @default.
• W2054029713 hasLocation W20540297134 @default.
• W2054029713 hasOpenAccess W2054029713 @default.
• W2054029713 hasPrimaryLocation W20540297131 @default.
• W2054029713 hasRelatedWork W1005409128 @default.
• W2054029713 hasRelatedWork W104535245 @default.
• W2054029713 hasRelatedWork W2048460395 @default.
• W2054029713 hasRelatedWork W2051640940 @default.
• W2054029713 hasRelatedWork W2052491883 @default.
• W2054029713 hasRelatedWork W2054029713 @default.
• W2054029713 hasRelatedWork W2056696427 @default.
• W2054029713 hasRelatedWork W2943474801 @default.
• W2054029713 hasRelatedWork W3168605014 @default.
• W2054029713 hasRelatedWork W4206108068 @default.
• W2054029713 isParatext "false" @default.
• W2054029713 isRetracted "false" @default.
• W2054029713 magId "2054029713" @default.
• W2054029713 workType "article" @default.