FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2054120161> ?p ?o ?g. }

• W2054120161 endingPage "300" @default.
• W2054120161 startingPage "288" @default.
• W2054120161 abstract "Recirculation following 2 h of focal ischemia due to transient middle cerebral artery (MCA) occlusion has previously been found to be accompanied by an initial, partial recovery of the cellular bioenergetic state and of mitochondrial respiratory functions, with secondary deterioration during the first 2-4 h of reflow. Both the free radical spin trap alpha-phenyl-N-tert-butyl nitrone (PBN) and the immunosuppressant drug FK506 ameliorate the damage incurred by the 2-h period of focal ischemia, even when given 1-3 h after the start of the recirculation. The primary objective of this study was to find out if FK506, like PBN, prevents the secondary deterioration of mitochondrial function, as this can be studied in vitro. Since this proved to be the case, we addressed the question of whether the secondary mitochondrial dysfunction and bioenergetic failure were related to a secondary compromise of microcirculation and cellular oxygen delivery. Six groups of male Wistar rats were studied for measurement of mitochondrial respiratory activity (total, n = 36). One group was used as control (n = 6). In the other groups of animals, MCA occlusion of 2 h duration was induced by an intraluminal filament technique, Neocortical focal and perifocal (penumbra) tissues were sampled after 2 h of ischemia (n = 6) and after 1 h (n = 6), 2 h (n = 6 with vehicle), and 4 h (n = 6 with vehicle; n = 6 with FK506) of recirculation. The vehicle or 1.0 mg.kg-1 of FK506 was injected intravenously after 1 h of recirculation. Homogenates were prepared, and stimulated (+ADP), nonstimulated (-ADP), and uncoupled respiratory rates were measured polarographically. The uncoupling agent used was carbonyl cyanide m-chlorophenylhydrazone. Local CBF and tissue oxygen tension were evaluated by laser-Doppler flowmetry and PO2 microelectrodes, respectively, throughout the whole periods of 2 h of ischemia and 4 h of recirculation, using a remote MCA occlusion technique. After 2 h of ischemia, the penumbra showed a moderate decrease and the focus a marked decrease in ADP-stimulated and uncoupled respiratory rates, with a marked fall in the respiratory control ratio, defined as ADP-stimulated divided by nonstimulated respiration. Recirculation (1 h) brought about partial recovery, but continued reflow (2 and 4 h) was associated with a secondary deterioration of respiratory functions. The secondary deterioration was prevented by FK506. The results thus confirm previous findings showing that secondary mitochondrial dysfunction occurs following transient focal cerebral ischemia and demonstrate that FK506, like PBN, improves the in vitro performance of mitochondria in focal and penumbral areas. Following MCA occlusion, local CBF in a penumbral area and tissue PO2 in a focal area decreased to about 30 and 5% of control, respectively. However, recirculation brought about rapid recovery of blood flow and oxygen delivery. During the whole 4-h period of recirculation, local CBF and tissue PO2 were maintained close to 100% and at about 160% of the preischemic level, respectively. The results make it highly unlikely that the secondary bioenergetic failure during recirculation is due to a compromised microcirculation. It follows that oxygen delivery is not rate-limiting for recovery events. Very likely, FK506 (and PBN) acts at the cellular level to improve mitochondrial energy functions." @default.
• W2054120161 created "2016-06-24" @default.
• W2054120161 creator A5004693769 @default.
• W2054120161 creator A5047457026 @default.
• W2054120161 creator A5052204317 @default.
• W2054120161 creator A5059402016 @default.
• W2054120161 date "1997-01-01" @default.
• W2054120161 modified "2023-10-18" @default.
• W2054120161 title "The Immunosuppressant Drug FK506 Ameliorates Secondary Mitochondrial Dysfunction Following Transient Focal Cerebral Ischemia in the Rat" @default.
• W2054120161 cites W150246076 @default.
• W2054120161 cites W1562075782 @default.
• W2054120161 cites W162447880 @default.
• W2054120161 cites W1728066272 @default.
• W2054120161 cites W1775749144 @default.
• W2054120161 cites W1818265222 @default.
• W2054120161 cites W1909841186 @default.
• W2054120161 cites W1964187920 @default.
• W2054120161 cites W1964826110 @default.
• W2054120161 cites W1969004566 @default.
• W2054120161 cites W1969612832 @default.
• W2054120161 cites W1969761846 @default.
• W2054120161 cites W1970190338 @default.
• W2054120161 cites W1971141628 @default.
• W2054120161 cites W1971335269 @default.
• W2054120161 cites W1972101997 @default.
• W2054120161 cites W1972681977 @default.
• W2054120161 cites W1974135000 @default.
• W2054120161 cites W1974182326 @default.
• W2054120161 cites W1976436058 @default.
• W2054120161 cites W1976541392 @default.
• W2054120161 cites W1976678881 @default.
• W2054120161 cites W1985221375 @default.
• W2054120161 cites W1988881255 @default.
• W2054120161 cites W1990695716 @default.
• W2054120161 cites W1994105347 @default.
• W2054120161 cites W1996786345 @default.
• W2054120161 cites W1996867825 @default.
• W2054120161 cites W1998025452 @default.
• W2054120161 cites W2007005170 @default.
• W2054120161 cites W2008757109 @default.
• W2054120161 cites W2010758846 @default.
• W2054120161 cites W2012763790 @default.
• W2054120161 cites W2015583712 @default.
• W2054120161 cites W2016871397 @default.
• W2054120161 cites W2017546932 @default.
• W2054120161 cites W2020362442 @default.
• W2054120161 cites W2020683010 @default.
• W2054120161 cites W2022443453 @default.
• W2054120161 cites W2022593257 @default.
• W2054120161 cites W2028097000 @default.
• W2054120161 cites W2029404346 @default.
• W2054120161 cites W2029706669 @default.
• W2054120161 cites W2033573399 @default.
• W2054120161 cites W2034528524 @default.
• W2054120161 cites W2034827296 @default.
• W2054120161 cites W2035838366 @default.
• W2054120161 cites W2037065721 @default.
• W2054120161 cites W2044435122 @default.
• W2054120161 cites W2045585355 @default.
• W2054120161 cites W2045698094 @default.
• W2054120161 cites W2047053137 @default.
• W2054120161 cites W2047362612 @default.
• W2054120161 cites W2049189117 @default.
• W2054120161 cites W2054712846 @default.
• W2054120161 cites W2054989425 @default.
• W2054120161 cites W2055183364 @default.
• W2054120161 cites W2055524735 @default.
• W2054120161 cites W2056824541 @default.
• W2054120161 cites W2057547483 @default.
• W2054120161 cites W2058432792 @default.
• W2054120161 cites W2063772445 @default.
• W2054120161 cites W2067436359 @default.
• W2054120161 cites W2069727032 @default.
• W2054120161 cites W2071365883 @default.
• W2054120161 cites W2072700178 @default.
• W2054120161 cites W2074036818 @default.
• W2054120161 cites W2074187719 @default.
• W2054120161 cites W2077921614 @default.
• W2054120161 cites W2079142476 @default.
• W2054120161 cites W2079452596 @default.
• W2054120161 cites W2081976133 @default.
• W2054120161 cites W2082870893 @default.
• W2054120161 cites W2084926712 @default.
• W2054120161 cites W2085935168 @default.
• W2054120161 cites W2089287517 @default.
• W2054120161 cites W2090233706 @default.
• W2054120161 cites W2094038479 @default.
• W2054120161 cites W2097307735 @default.
• W2054120161 cites W2098712005 @default.
• W2054120161 cites W2100358219 @default.
• W2054120161 cites W2101575450 @default.
• W2054120161 cites W2125683177 @default.
• W2054120161 cites W2131461587 @default.
• W2054120161 cites W2144437860 @default.
• W2054120161 cites W2154346380 @default.
• W2054120161 cites W2155747798 @default.
• W2054120161 cites W2409468262 @default.
• W2054120161 cites W4211255027 @default.

• next