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- W2054144413 abstract "Slurry-packed fused-silica microcolumns of 250 micron I.D., are characterized for use in high-performance liquid chromatographic studies of proteins. The present work utilizes the reversed-phase and size-exclusion chromatographic modes for the separation of standard protein mixtures. A 5-micron, 300-A octyl material is utilized for the reversed-phase studies, and the size-exclusion studies are accomplished with 5-micron diol material of 60-, 100- and 300-A pore sizes. Column efficiency and packing reproducibility, as well as sample capacity in a micropreparative mode, are discussed. In addition, the inherent mass sensitivity of a microcolumn liquid chromatography system as applied to protein detection is demonstrated." @default.
- W2054144413 created "2016-06-24" @default.
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- W2054144413 date "1988-01-01" @default.
- W2054144413 modified "2023-10-16" @default.
- W2054144413 title "Separation of proteins by microcolumn liquid chromatography based on the reversed-phase and size-exclusion principles" @default.
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- W2054144413 doi "https://doi.org/10.1016/s0021-9673(01)84566-0" @default.
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