FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2054148967> ?p ?o ?g. }

• W2054148967 endingPage "112" @default.
• W2054148967 startingPage "101" @default.
• W2054148967 abstract "Proteins often exist as ensembles of interconverting states in solution which are often difficult to quantify. In the present manuscript we show that the combination of MS under nondenaturing conditions and AUC-SV (analytical ultracentrifugation sedimentation velocity) unambiguously clarifies a distribution of states and hydrodynamic shapes of assembled oligomers for the NAP-1 (nucleosome assembly protein 1). MS established the number of associated units, which was utilized as input for the numerical analysis of AUC-SV profiles. The AUC-SV analysis revealed that less than 1% of NAP-1 monomer exists at the micromolar concentration range and that the basic assembly unit consists of dimers of yeast or human NAP-1. These dimers interact non-covalently to form even-numbered higher-assembly states, such as tetramers, hexamers, octamers and decamers. MS and AUC-SV consistently showed that the formation of the higher oligomers was suppressed with increasing ionic strength, implicating electrostatic interactions in the formation of higher oligomers. The hydrodynamic shapes of the NAP-1 tetramer estimated from AUC-SV agreed with the previously proposed assembly models built using the known three-dimensional structure of yeast NAP-1. Those of the hexamer and octamer could be represented by new models shown in the present study. Additionally, MS was used to measure the stoichiometry of the interaction between the human NAP-1 dimer and the histone H2A–H2B dimer or H3–H4 tetramer. The present study illustrates a rigorous procedure for the analysis of protein assembly and protein–protein interactions in solution." @default.
• W2054148967 created "2016-06-24" @default.
• W2054148967 creator A5001323567 @default.
• W2054148967 creator A5003402066 @default.
• W2054148967 creator A5012445152 @default.
• W2054148967 creator A5013798827 @default.
• W2054148967 creator A5024888646 @default.
• W2054148967 creator A5026448810 @default.
• W2054148967 creator A5027851091 @default.
• W2054148967 creator A5032080185 @default.
• W2054148967 creator A5035478723 @default.
• W2054148967 creator A5045930049 @default.
• W2054148967 creator A5049969139 @default.
• W2054148967 creator A5052713136 @default.
• W2054148967 creator A5063736660 @default.
• W2054148967 creator A5084323300 @default.
• W2054148967 date "2011-04-27" @default.
• W2054148967 modified "2023-09-26" @default.
• W2054148967 title "Assembly states of the nucleosome assembly protein 1 (NAP-1) revealed by sedimentation velocity and non-denaturing MS" @default.
• W2054148967 cites W1523954091 @default.
• W2054148967 cites W1571482483 @default.
• W2054148967 cites W1612183828 @default.
• W2054148967 cites W1907262042 @default.
• W2054148967 cites W1924576234 @default.
• W2054148967 cites W1965701922 @default.
• W2054148967 cites W1975317507 @default.
• W2054148967 cites W1977027474 @default.
• W2054148967 cites W1989406431 @default.
• W2054148967 cites W1992446000 @default.
• W2054148967 cites W1998355675 @default.
• W2054148967 cites W2009238064 @default.
• W2054148967 cites W2019349381 @default.
• W2054148967 cites W2031603364 @default.
• W2054148967 cites W2044615030 @default.
• W2054148967 cites W2053802188 @default.
• W2054148967 cites W2053955463 @default.
• W2054148967 cites W2055142162 @default.
• W2054148967 cites W2063516501 @default.
• W2054148967 cites W2063607742 @default.
• W2054148967 cites W2066011752 @default.
• W2054148967 cites W2080849703 @default.
• W2054148967 cites W2091352351 @default.
• W2054148967 cites W2093214796 @default.
• W2054148967 cites W2097074036 @default.
• W2054148967 cites W2097365353 @default.
• W2054148967 cites W2098106514 @default.
• W2054148967 cites W2118214304 @default.
• W2054148967 cites W2121996872 @default.
• W2054148967 cites W2124059113 @default.
• W2054148967 cites W2127316404 @default.
• W2054148967 cites W2129378146 @default.
• W2054148967 cites W2144792857 @default.
• W2054148967 cites W2156947774 @default.
• W2054148967 cites W2165521391 @default.
• W2054148967 cites W2167499262 @default.
• W2054148967 cites W2169145132 @default.
• W2054148967 cites W2169311669 @default.
• W2054148967 cites W4247418383 @default.
• W2054148967 doi "https://doi.org/10.1042/bj20102063" @default.
• W2054148967 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21348863" @default.
• W2054148967 hasPublicationYear "2011" @default.
• W2054148967 type Work @default.
• W2054148967 sameAs 2054148967 @default.
• W2054148967 citedByCount "20" @default.
• W2054148967 countsByYear W20541489672012 @default.
• W2054148967 countsByYear W20541489672013 @default.
• W2054148967 countsByYear W20541489672014 @default.
• W2054148967 countsByYear W20541489672015 @default.
• W2054148967 countsByYear W20541489672016 @default.
• W2054148967 countsByYear W20541489672017 @default.
• W2054148967 countsByYear W20541489672018 @default.
• W2054148967 countsByYear W20541489672019 @default.
• W2054148967 countsByYear W20541489672020 @default.
• W2054148967 countsByYear W20541489672023 @default.
• W2054148967 crossrefType "journal-article" @default.
• W2054148967 hasAuthorship W2054148967A5001323567 @default.
• W2054148967 hasAuthorship W2054148967A5003402066 @default.
• W2054148967 hasAuthorship W2054148967A5012445152 @default.
• W2054148967 hasAuthorship W2054148967A5013798827 @default.
• W2054148967 hasAuthorship W2054148967A5024888646 @default.
• W2054148967 hasAuthorship W2054148967A5026448810 @default.
• W2054148967 hasAuthorship W2054148967A5027851091 @default.
• W2054148967 hasAuthorship W2054148967A5032080185 @default.
• W2054148967 hasAuthorship W2054148967A5035478723 @default.
• W2054148967 hasAuthorship W2054148967A5045930049 @default.
• W2054148967 hasAuthorship W2054148967A5049969139 @default.
• W2054148967 hasAuthorship W2054148967A5052713136 @default.
• W2054148967 hasAuthorship W2054148967A5063736660 @default.
• W2054148967 hasAuthorship W2054148967A5084323300 @default.
• W2054148967 hasConcept C104317684 @default.
• W2054148967 hasConcept C107513589 @default.
• W2054148967 hasConcept C12554922 @default.
• W2054148967 hasConcept C166940927 @default.
• W2054148967 hasConcept C169760540 @default.
• W2054148967 hasConcept C173681060 @default.
• W2054148967 hasConcept C178790620 @default.
• W2054148967 hasConcept C181199279 @default.
• W2054148967 hasConcept C185592680 @default.

• next