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- W2054164967 abstract "We have developed a rapid and efficient expression system to study the human β 2 adrenergic receptor (huβ 2AR) in the fission yeast Schizosaccharomyces pombe. This was achieved by cloning the huβ 2AR gene, modified by replacement of the 5′ untranslated and a small part of the N-terminal coding sequence (first 14 amino acids) with the corresponding region of the yeast Saccharomyces cerevisiae STE2 (α-factor receptor) gene. The gene was then placed under the control of a S. pombe constitutive promoter for alcohol dehydrogenase (adh). Huβ 2AR expression was assessed by immunoblot analysis of the chimeric protein with an anti-STE2 serum raised against a dodecapeptide homologous to the N-terminal amino acids of STE2 and ligand binding was assayed using [125I]cyanopindolol. We demonstrate here that the chimeric receptor expressed in S. pombe exhibits the same characteristic ligand specificity and affinity as that of the authentic huβ 2AR. This system constitutes a convenient alternative to existing methods for studying seven transmembrane domain receptors due to its simplicity and high reproducibility." @default.
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- W2054164967 date "1995-12-18" @default.
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- W2054164967 title "The humanβ2-adrenergic receptor expressed inSchizosaccharomyces pomberetains its pharmacological properties" @default.
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- W2054164967 doi "https://doi.org/10.1016/0014-5793(95)01330-x" @default.
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