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• W2054165004 abstract "Different conformations have been identified for the enzyme valyl-tRNA synthetase from yeast inside its complex with one tRNA molecule by neutron scattering. One form is identical to that of the free enzyme in solution; the other form is more contracted, having a radius of gyration which is smaller by 10% and a specific volume which is smaller by 1%. The contracted conformation has been found for the complexes with tRNAVal and tRNAAsp in phosphate buffer (pH 6.3) provided the ionic strength is lower than about 150 mm. In higher ionic strength (up to about 500 mm) the enzyme still forms a complex with tRNAVal but its conformation remains that of the free protein in solution. In the complex with tRNA3Leu, the enzyme conformation is that of the free state even at the lowest ionic strength examined (that of the phosphate buffer, 60 mm). The free enzyme is an elongated molecule of radius of gyration 40 Å (a compact protein of the same molecular weight would have a radius of gyration of 30 Å). The positioning within the complex of tRNAVal, on the one hand, and tRNA3Leu, on the other, is very different. The first tRNA is intimately associated with the enzyme, lying predominantly closer to the centre of mass of the complex than the protein. In the complex with tRNA3Leu, the tRNA lies further away from the centre of mass of the complex than the protein. Small concentrations of tRNAVal, tRNAAsp, tRNA3Leu or Escherichia coli 5 S ribosomal RNA cause the enzyme to aggregate into dimers, trimers and higher aggregates provided the ionic strength of the buffer is below 150 mm. In higher ionic strength or for [RNA]: [enzyme] > 1 the aggregates are dissociated to yield the one-to-one RNA-enzyme complex." @default.
• W2054165004 created "2016-06-24" @default.
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• W2054165004 date "1979-04-01" @default.
• W2054165004 modified "2023-10-17" @default.
• W2054165004 title "Interactions of yeast valyl-tRNA synthetase with RNAs and conformational changes of the enzyme" @default.
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• W2054165004 doi "https://doi.org/10.1016/0022-2836(79)90508-4" @default.
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