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- W2054204920 abstract "The currently available methods for estimating the absorption of dietary cholesterol are so difficult that only a few studies under rather restricted experimental conditions have been conducted. A very simple approach circumventing these difficulties is based on the determination of two isotopes (3H and 14C) in a single unmeasured “test” sample of feces after feeding a mixture of cholesterol-1-2-3H (5 μCi), β-sitosterol-4-14C (1 μCi) and carmine red (300 mg). The two sterols undergo similar conversions and degradations in the gastrointestinal tract, except for the differences in their absorption. Thus, the fraction of dietary cholesterol which is absorbed in excess of the absorption of β-sitosterol can be calculated merely from the difference in the 3H/14C ratios between the mixture given by mouth and that obtained from the feces. It is important to examine the first sample of feces containing the red color of the carmine red since this “test” sample contains the unabsorbed sterols uncontaminated by radioactivity from the biliary cholesterol and bile acids." @default.
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- W2054204920 date "1971-05-01" @default.
- W2054204920 modified "2023-09-23" @default.
- W2054204920 title "A Simple Method for Calculating Absorption of Dietary Cholesterol in Man" @default.
- W2054204920 doi "https://doi.org/10.3181/00379727-137-35560" @default.
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