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• W2054207955 startingPage "708" @default.
• W2054207955 abstract "Microcystins are a major group of cyanobacterial heptapeptide toxins found in freshwater and brackish environments. There is currently an urgent requirement for highly-sensitive, rapid and in-expensive detection methodologies for these toxins. A novel single chain fragment variable (scFv) fragment was generated and is the first known report of a recombinant anti-microcystin avian antibody. In a surface plasmon resonance-based immunoassay, the antibody fragment displayed cross-reactivity with seven microcystin congeners (microcystin–leucine–arginine (MC-LR) 100%, microcystin–tyrosine–arginine (MC-YR) 79.7%, microcystin–leucine–alanine (MC-LA) 74.8%, microcystin–leucine–phenylalanine (MC-LF) 67.5%, microcystin–leucine–tryptophan (MC-LW) 63.7%, microcystin–arginine–arginine (MC-RR) 60.1% and nodularin (Nod) 69.3%, % cross reactivity). Following directed molecular evolution of the parental clone the resultant affinity-enhanced antibody fragment was applied in an optimized fluorescence immunoassay on a planar waveguide detection system. This novel immuno-sensing format can detect free microcystin-LR with a functional limit of detection of 0.19 ng mL−1and a detection range of 0.21–5.9 ng mL−1. The assay is highly reproducible (displaying percentage coefficients of variance below 8% for intra-day assays and below 11% for inter-day assays), utilizes an inexpensive cartridge system with low reagent volumes and can be completed in less than twenty minutes." @default.
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• W2054207955 date "2015-05-01" @default.
• W2054207955 modified "2023-10-10" @default.
• W2054207955 title "Detection of the cyanobacterial toxin, microcystin-LR, using a novel recombinant antibody-based optical-planar waveguide platform" @default.
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• W2054207955 doi "https://doi.org/10.1016/j.bios.2014.10.039" @default.
• W2054207955 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25459059" @default.
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