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• W2054276833 abstract "<b>Background:</b> Transforming growth factor beta (TGFβ) upregulates a number of smooth muscle specific genes in (myo)fibroblasts. As asthma is characterised by an increase in airway smooth muscle, we postulated that TGFβ₂ favours differentiation of asthmatic (myo)fibroblasts towards a smooth muscle phenotype. <b>Methods:</b> Primary fibroblasts were grown from bronchial biopsy specimens from normal (n = 6) and asthmatic (n = 7) donors and treated with TGFβ₂ to induce myofibroblast differentiation. The most stable genes for normalisation were identified using RT-qPCR and the geNorm software applied to a panel of 12 “housekeeping” genes. Expression of α-smooth muscle actin (αSMA), heavy chain myosin (HCM), calponin 1 (CPN 1), desmin, and γ-actin were measured by RT-qPCR. Protein expression was assessed by immunocytochemistry and western blotting. <b>Results:</b> Phospholipase A2 and ubiquitin C were identified as the most stably expressed and practically useful genes for normalisation of gene expression during myofibroblast differentiation. TGFβ₂ induced mRNA expression for all five smooth muscle related transcripts; αSMA, HCM and CPN 1 protein were also increased but desmin protein was not detectable. Although there was no difference in basal expression, HCM, CPN 1 and desmin were induced to a significantly greater extent in asthmatic fibroblasts than in those from normal controls (p = 0.041 and 0.011, respectively). <b>Conclusions:</b> Although TGFβ₂ induced the transcription of several smooth muscle related genes, not all were translated into protein. Thus, while TGFβ₂ is unable to induce a bona fide smooth muscle cell phenotype, it may “prime” (myo)fibroblasts for further differentiation, especially if the cells are derived from asthmatic airways." @default.
• W2054276833 created "2016-06-24" @default.
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• W2054276833 date "2006-04-01" @default.
• W2054276833 modified "2023-09-25" @default.
• W2054276833 title "Enhanced upregulation of smooth muscle related transcripts by TGF 2 in asthmatic (myo) fibroblasts" @default.
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• W2054276833 doi "https://doi.org/10.1136/thx.2005.050005" @default.
• W2054276833 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2104615" @default.
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