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• W2054278139 abstract "We have established an erythropoietin-dependent human leukemia cell line, AS-E2, from a patient with acute myeloid leukemia. These cells have many characteristics of late erythroid progenitor cells, they are positive for CD36, Glycophorin A, and CD71 but negative for CD41, and positive for benzidine and PAS staining. These cells express GATA-1 and have low affinity erythropoietin (EPO) receptor on their surface. Interestingly, AS-E2 cells are strictly dependent on EPO for their growth and survival; other cytokines including GM-CSF, stem cell factor, or IL-3 cannot support the growth of this cell line. These features are similar to late erythroid lineage cells, like normal BFU-E or CFU-E, and we have demonstrated that EPO stimulation induces the tyrosine phosphorylation of several proteins in AS-E2 cells including the EPO receptor and JAK2 kinase. This new cell line is a useful reagent to study biological and molecular events during the late stages of erythropoiesis, and to understand transforming events in human erythroid cells." @default.
• W2054278139 created "2016-06-24" @default.
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• W2054278139 date "1997-11-01" @default.
• W2054278139 modified "2023-10-15" @default.
• W2054278139 title "Establishment and characterization of a new erythropoietin-dependent acute myeloid leukemia cell line, AS-E2" @default.
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• W2054278139 doi "https://doi.org/10.1038/sj.leu.2400838" @default.
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