FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2054295839> ?p ?o ?g. }

• W2054295839 endingPage "348" @default.
• W2054295839 startingPage "336" @default.
• W2054295839 abstract "Regulator of G Protein Signalling (RGS) proteins impede heterotrimeric G protein signalling. RGS2 decreases cAMP production and appears to interact with both adenylyl cyclase (AC) and its stimulatory G protein Gs. We showed previously that Green Fluorescent Protein-tagged RGS2 (GFP-RGS2) localizes to the nucleus in HEK 293 cells and is recruited to the plasma membrane when co-expressed with Gsα, or the Gs-coupled β2-adrenergic receptor (β2AR). Here, using confocal microscopy we show that co-expression of various AC isoforms (ACI, ACII, ACV, ACVI) also leads to GFP-RGS2 recruitment to the plasma membrane. Bioluminescence Resonance Energy Transfer (BRET) was also used to examine physical interactions between RGS2 and components of the Gs-signalling pathway. A BRET signal was detected between fusion constructs of RGS2-Renilla luciferase (energy donor) and Gsα-GFP (energy acceptor) co-expressed in HEK 293 cells. BRET was also observed between GFP-RGS2 and ACII or ACVI fused to Renilla luciferase. Additionally, RGS2 was found to interact with the β2AR. Purified RGS2 selectively bound to the third intracellular loop of the β2AR in GST pulldown assays, and a BRET signal was observed between GFP-RGS2 and β2AR fused to Renilla luciferase when these two proteins were co-expressed together with either ACIV or ACVI. This interaction was below the limit of detection in the absence of co-expressed AC, suggesting that the effector enzyme stabilized or promoted binding between the receptor and the RGS protein inside the cell. Taken together, these results suggest the possibility that RGS2 might bind to a receptor-G protein-effector signalling complex to regulate Gs-dependent cAMP production." @default.
• W2054295839 created "2016-06-24" @default.
• W2054295839 creator A5001113992 @default.
• W2054295839 creator A5015897966 @default.
• W2054295839 creator A5027967765 @default.
• W2054295839 creator A5052328017 @default.
• W2054295839 creator A5067295524 @default.
• W2054295839 creator A5083215596 @default.
• W2054295839 date "2006-03-01" @default.
• W2054295839 modified "2023-10-16" @default.
• W2054295839 title "RGS2 interacts with Gs and adenylyl cyclase in living cells" @default.
• W2054295839 cites W1543575625 @default.
• W2054295839 cites W1547325917 @default.
• W2054295839 cites W1953109900 @default.
• W2054295839 cites W1985329934 @default.
• W2054295839 cites W1999797349 @default.
• W2054295839 cites W2002154523 @default.
• W2054295839 cites W2004962395 @default.
• W2054295839 cites W2010357110 @default.
• W2054295839 cites W2011276907 @default.
• W2054295839 cites W201193444 @default.
• W2054295839 cites W2012043177 @default.
• W2054295839 cites W2016489131 @default.
• W2054295839 cites W2017663972 @default.
• W2054295839 cites W2023410491 @default.
• W2054295839 cites W2023649595 @default.
• W2054295839 cites W2025932643 @default.
• W2054295839 cites W2035548897 @default.
• W2054295839 cites W2039371098 @default.
• W2054295839 cites W2040896718 @default.
• W2054295839 cites W2041138404 @default.
• W2054295839 cites W2042937027 @default.
• W2054295839 cites W2044570582 @default.
• W2054295839 cites W2044585881 @default.
• W2054295839 cites W2048351462 @default.
• W2054295839 cites W2049174331 @default.
• W2054295839 cites W2058554459 @default.
• W2054295839 cites W2058801184 @default.
• W2054295839 cites W2060536455 @default.
• W2054295839 cites W2076311461 @default.
• W2054295839 cites W2079239816 @default.
• W2054295839 cites W2088712681 @default.
• W2054295839 cites W2106178916 @default.
• W2054295839 cites W2116905625 @default.
• W2054295839 cites W2126102214 @default.
• W2054295839 cites W2130283831 @default.
• W2054295839 cites W2136972830 @default.
• W2054295839 cites W2147198454 @default.
• W2054295839 cites W2153076718 @default.
• W2054295839 cites W2164537261 @default.
• W2054295839 cites W4249976428 @default.
• W2054295839 doi "https://doi.org/10.1016/j.cellsig.2005.05.004" @default.
• W2054295839 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16095880" @default.
• W2054295839 hasPublicationYear "2006" @default.
• W2054295839 type Work @default.
• W2054295839 sameAs 2054295839 @default.
• W2054295839 citedByCount "110" @default.
• W2054295839 countsByYear W20542958392012 @default.
• W2054295839 countsByYear W20542958392013 @default.
• W2054295839 countsByYear W20542958392014 @default.
• W2054295839 countsByYear W20542958392015 @default.
• W2054295839 countsByYear W20542958392016 @default.
• W2054295839 countsByYear W20542958392017 @default.
• W2054295839 countsByYear W20542958392018 @default.
• W2054295839 countsByYear W20542958392019 @default.
• W2054295839 countsByYear W20542958392020 @default.
• W2054295839 countsByYear W20542958392022 @default.
• W2054295839 countsByYear W20542958392023 @default.
• W2054295839 crossrefType "journal-article" @default.
• W2054295839 hasAuthorship W2054295839A5001113992 @default.
• W2054295839 hasAuthorship W2054295839A5015897966 @default.
• W2054295839 hasAuthorship W2054295839A5027967765 @default.
• W2054295839 hasAuthorship W2054295839A5052328017 @default.
• W2054295839 hasAuthorship W2054295839A5067295524 @default.
• W2054295839 hasAuthorship W2054295839A5083215596 @default.
• W2054295839 hasConcept C104317684 @default.
• W2054295839 hasConcept C111335760 @default.
• W2054295839 hasConcept C139407321 @default.
• W2054295839 hasConcept C142613039 @default.
• W2054295839 hasConcept C153911025 @default.
• W2054295839 hasConcept C163181125 @default.
• W2054295839 hasConcept C170493617 @default.
• W2054295839 hasConcept C174510640 @default.
• W2054295839 hasConcept C176303035 @default.
• W2054295839 hasConcept C185592680 @default.
• W2054295839 hasConcept C2779178603 @default.
• W2054295839 hasConcept C4963665 @default.
• W2054295839 hasConcept C54009773 @default.
• W2054295839 hasConcept C55493867 @default.
• W2054295839 hasConcept C62478195 @default.
• W2054295839 hasConcept C80631254 @default.
• W2054295839 hasConcept C86803240 @default.
• W2054295839 hasConcept C95444343 @default.
• W2054295839 hasConceptScore W2054295839C104317684 @default.
• W2054295839 hasConceptScore W2054295839C111335760 @default.
• W2054295839 hasConceptScore W2054295839C139407321 @default.
• W2054295839 hasConceptScore W2054295839C142613039 @default.
• W2054295839 hasConceptScore W2054295839C153911025 @default.

• next