Matches in SemOpenAlex for { <https://semopenalex.org/work/W2054303941> ?p ?o ?g. }
- W2054303941 endingPage "922" @default.
- W2054303941 startingPage "914" @default.
- W2054303941 abstract "Rat glomerular epithelial cells produce and bear factor H on their surface that is up-regulated under complement attack.BackgroundFactor H is a potent complement inhibitory molecule that is primarily produced by the liver and appears in plasma as a soluble protein. Yet there is evidence that other cells, including those in the kidney, can produce factor H, and that it can be cell-associated as well as present as a plasma protein. Here we studied factor H in rat glomerular epithelial cells (GEC).MethodsA polyclonal antibody to factor H was used to identify factor H protein. A polymerase chain reaction (PCR)-based strategy was utilized to clone the full-length cDNA of GEC factor H. The relative quantity of factor H mRNA was measured by quantitative reverse transcription (RT)-PCR in cultured GEC exposed to complement activation and in the passive Heymann nephritis (PHN) model of membranous nephropathy.ResultsBy immunofluorescence microscopy, factor H protein was present on the plasma membranes of cultured GEC. Based upon Western blot studies, this appeared to be the full-length 150 kD factor H protein. Factor H cDNA cloned from GEC was identical to the newly deposited sequence for rat liver factor H cDNA. In cultured GEC in which complement was activated, factor H mRNA increased over time. Similarly, in the PHN model in which complement was activated on GEC in vivo, factor H mRNA and protein also increased over time.ConclusionCultured GEC and glomeruli express factor H mRNA and protein. As modeled both in vitro and in vivo in the rat, factor H is up-regulated in membranous nephropathy. This is likely to be a direct response of GEC to complement attack and may represent a protective response of this cell. Rat glomerular epithelial cells produce and bear factor H on their surface that is up-regulated under complement attack. Factor H is a potent complement inhibitory molecule that is primarily produced by the liver and appears in plasma as a soluble protein. Yet there is evidence that other cells, including those in the kidney, can produce factor H, and that it can be cell-associated as well as present as a plasma protein. Here we studied factor H in rat glomerular epithelial cells (GEC). A polyclonal antibody to factor H was used to identify factor H protein. A polymerase chain reaction (PCR)-based strategy was utilized to clone the full-length cDNA of GEC factor H. The relative quantity of factor H mRNA was measured by quantitative reverse transcription (RT)-PCR in cultured GEC exposed to complement activation and in the passive Heymann nephritis (PHN) model of membranous nephropathy. By immunofluorescence microscopy, factor H protein was present on the plasma membranes of cultured GEC. Based upon Western blot studies, this appeared to be the full-length 150 kD factor H protein. Factor H cDNA cloned from GEC was identical to the newly deposited sequence for rat liver factor H cDNA. In cultured GEC in which complement was activated, factor H mRNA increased over time. Similarly, in the PHN model in which complement was activated on GEC in vivo, factor H mRNA and protein also increased over time. Cultured GEC and glomeruli express factor H mRNA and protein. As modeled both in vitro and in vivo in the rat, factor H is up-regulated in membranous nephropathy. This is likely to be a direct response of GEC to complement attack and may represent a protective response of this cell." @default.
- W2054303941 created "2016-06-24" @default.
- W2054303941 creator A5024189919 @default.
- W2054303941 creator A5043487482 @default.
- W2054303941 creator A5055520859 @default.
- W2054303941 creator A5071999370 @default.
- W2054303941 creator A5087485954 @default.
- W2054303941 date "2003-09-01" @default.
- W2054303941 modified "2023-10-06" @default.
- W2054303941 title "Rat glomerular epithelial cells produce and bear factor H on their surface that is up-regulated under complement attack" @default.
- W2054303941 cites W1036732202 @default.
- W2054303941 cites W1483924912 @default.
- W2054303941 cites W1500833770 @default.
- W2054303941 cites W1503484093 @default.
- W2054303941 cites W1542975757 @default.
- W2054303941 cites W1564844664 @default.
- W2054303941 cites W1756973693 @default.
- W2054303941 cites W1932028861 @default.
- W2054303941 cites W1963905188 @default.
- W2054303941 cites W1973218559 @default.
- W2054303941 cites W1976362917 @default.
- W2054303941 cites W1980344266 @default.
- W2054303941 cites W1983588293 @default.
- W2054303941 cites W1988130433 @default.
- W2054303941 cites W1996115257 @default.
- W2054303941 cites W1996697580 @default.
- W2054303941 cites W1998880568 @default.
- W2054303941 cites W2001579563 @default.
- W2054303941 cites W2009117850 @default.
- W2054303941 cites W2014115469 @default.
- W2054303941 cites W2021826494 @default.
- W2054303941 cites W2022838108 @default.
- W2054303941 cites W2028478459 @default.
- W2054303941 cites W2045840718 @default.
- W2054303941 cites W2048681283 @default.
- W2054303941 cites W2050506138 @default.
- W2054303941 cites W2054003769 @default.
- W2054303941 cites W2054203856 @default.
- W2054303941 cites W2054374575 @default.
- W2054303941 cites W2060954256 @default.
- W2054303941 cites W2065853381 @default.
- W2054303941 cites W2069305908 @default.
- W2054303941 cites W2072417552 @default.
- W2054303941 cites W2073745575 @default.
- W2054303941 cites W2073917494 @default.
- W2054303941 cites W2075021230 @default.
- W2054303941 cites W2076406226 @default.
- W2054303941 cites W2083352207 @default.
- W2054303941 cites W2090273752 @default.
- W2054303941 cites W2094275695 @default.
- W2054303941 cites W2120467386 @default.
- W2054303941 cites W2124410251 @default.
- W2054303941 cites W2129778430 @default.
- W2054303941 cites W2136051502 @default.
- W2054303941 cites W2140972898 @default.
- W2054303941 cites W2142480343 @default.
- W2054303941 cites W2144932484 @default.
- W2054303941 cites W2158981826 @default.
- W2054303941 cites W2174272589 @default.
- W2054303941 cites W2223359185 @default.
- W2054303941 cites W2273511952 @default.
- W2054303941 cites W2346909892 @default.
- W2054303941 cites W2357066626 @default.
- W2054303941 cites W4313342402 @default.
- W2054303941 doi "https://doi.org/10.1046/j.1523-1755.2003.00188.x" @default.
- W2054303941 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12911541" @default.
- W2054303941 hasPublicationYear "2003" @default.
- W2054303941 type Work @default.
- W2054303941 sameAs 2054303941 @default.
- W2054303941 citedByCount "17" @default.
- W2054303941 countsByYear W20543039412013 @default.
- W2054303941 countsByYear W20543039412015 @default.
- W2054303941 countsByYear W20543039412016 @default.
- W2054303941 countsByYear W20543039412019 @default.
- W2054303941 countsByYear W20543039412022 @default.
- W2054303941 crossrefType "journal-article" @default.
- W2054303941 hasAuthorship W2054303941A5024189919 @default.
- W2054303941 hasAuthorship W2054303941A5043487482 @default.
- W2054303941 hasAuthorship W2054303941A5055520859 @default.
- W2054303941 hasAuthorship W2054303941A5071999370 @default.
- W2054303941 hasAuthorship W2054303941A5087485954 @default.
- W2054303941 hasBestOaLocation W20543039411 @default.
- W2054303941 hasConcept C104317684 @default.
- W2054303941 hasConcept C105580179 @default.
- W2054303941 hasConcept C111684460 @default.
- W2054303941 hasConcept C14356644 @default.
- W2054303941 hasConcept C153911025 @default.
- W2054303941 hasConcept C159654299 @default.
- W2054303941 hasConcept C179369057 @default.
- W2054303941 hasConcept C187882448 @default.
- W2054303941 hasConcept C203014093 @default.
- W2054303941 hasConcept C2776415932 @default.
- W2054303941 hasConcept C55493867 @default.
- W2054303941 hasConcept C8487232 @default.
- W2054303941 hasConcept C86803240 @default.
- W2054303941 hasConcept C956191 @default.
- W2054303941 hasConceptScore W2054303941C104317684 @default.
- W2054303941 hasConceptScore W2054303941C105580179 @default.