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- W2054304082 abstract "Methylmercury (MeHg) is a widespread environmental toxicant with major actions on the central nervous system. Among the neurons reportedly affected in cases of Hg poisoning are motor neurons; however, the direct cellular effects of MeHg on motor neurons have not been reported. Ratiometric fluorescence imaging, using the Ca2+-sensitive fluorophore fura-2, was used to examine the effect of MeHg on Ca2+ homeostasis in primary cultures of mouse spinal motor neurons. In vitro MeHg exposure at concentrations (0.1–2 μM) known to affect other neurons in culture differentially, induced a biphasic rise in fura-2 fluorescence ratio indicating an increase in [Ca2+]i. The time-to-onset of these fura-2 fluorescence ratio changes was inversely correlated with MeHg concentration. TPEN (20 μM), a non-Ca2+, divalent cation chelator, reduced the amplitude of the increase in fura-2 fluorescence induced by MeHg in the first phase, indicating that both Ca2+ and non-Ca2+ divalent cations contribute to the MeHg-induced effect. When examining various Ca2+ entry pathways as possible targets contributing to Ca2+ influx, we found that excitatory amino acid receptor blockers MK-801 (15 μM), and AP-5 (100 μM)—both NMDA receptor-operated ion channel blockers, CNQX (20 μM), a non-NMDA receptor blocker, and the voltage-dependent Ca2+ channel blockers nifedipine (1 μM) and ω-conotoxin-GVIA (1 μM) all significantly delayed the development of increased Ca2+ caused by MeHg. The voltage-dependent Na+ channel blocker tetrodotoxin (TTX, 1 μM) did not alter the MeHg-induced increases in fura-2 fluorescence ratio. Thus, MeHg alters Ca2+ homeostasis in mouse spinal motor neurons through excitatory amino acid receptor-mediated pathways, and nifedipine and ω-conotoxin-GVIA-sensitive pathways. Spinal motor neurons are highly sensitive to this effect of acute exposure to MeHg." @default.
- W2054304082 created "2016-06-24" @default.
- W2054304082 creator A5014156549 @default.
- W2054304082 creator A5041588481 @default.
- W2054304082 date "2011-12-01" @default.
- W2054304082 modified "2023-09-30" @default.
- W2054304082 title "Ca2+ entry pathways in mouse spinal motor neurons in culture following in vitro exposure to methylmercury" @default.
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- W2054304082 cites W1760737596 @default.
- W2054304082 cites W1916645464 @default.
- W2054304082 cites W1964428482 @default.
- W2054304082 cites W1966377830 @default.
- W2054304082 cites W1968442134 @default.
- W2054304082 cites W1970761857 @default.
- W2054304082 cites W1973275674 @default.
- W2054304082 cites W1973620200 @default.
- W2054304082 cites W1974795836 @default.
- W2054304082 cites W1980976762 @default.
- W2054304082 cites W1983423336 @default.
- W2054304082 cites W1985337462 @default.
- W2054304082 cites W1990578351 @default.
- W2054304082 cites W1997568797 @default.
- W2054304082 cites W1998078640 @default.
- W2054304082 cites W2009016920 @default.
- W2054304082 cites W2009160195 @default.
- W2054304082 cites W2010430324 @default.
- W2054304082 cites W2013220549 @default.
- W2054304082 cites W2016575029 @default.
- W2054304082 cites W2022375976 @default.
- W2054304082 cites W2024156553 @default.
- W2054304082 cites W2031568543 @default.
- W2054304082 cites W2031754719 @default.
- W2054304082 cites W2032176562 @default.
- W2054304082 cites W2032572133 @default.
- W2054304082 cites W2036769991 @default.
- W2054304082 cites W2040330912 @default.
- W2054304082 cites W2040606294 @default.
- W2054304082 cites W2041814921 @default.
- W2054304082 cites W2044480762 @default.
- W2054304082 cites W2048152070 @default.
- W2054304082 cites W2049949557 @default.
- W2054304082 cites W2052598520 @default.
- W2054304082 cites W2054501260 @default.
- W2054304082 cites W2054862715 @default.
- W2054304082 cites W2059075664 @default.
- W2054304082 cites W2061030418 @default.
- W2054304082 cites W2062225778 @default.
- W2054304082 cites W2067425399 @default.
- W2054304082 cites W2068546892 @default.
- W2054304082 cites W2068561089 @default.
- W2054304082 cites W2070178640 @default.
- W2054304082 cites W2075128291 @default.
- W2054304082 cites W2079127306 @default.
- W2054304082 cites W2084202107 @default.
- W2054304082 cites W2087969790 @default.
- W2054304082 cites W2091382819 @default.
- W2054304082 cites W2091481654 @default.
- W2054304082 cites W2094474445 @default.
- W2054304082 cites W2098998433 @default.
- W2054304082 cites W2102620386 @default.
- W2054304082 cites W2104343707 @default.
- W2054304082 cites W2105517036 @default.
- W2054304082 cites W2109249103 @default.
- W2054304082 cites W2118309627 @default.
- W2054304082 cites W2123476231 @default.
- W2054304082 cites W2127031191 @default.
- W2054304082 cites W2127964855 @default.
- W2054304082 cites W2132186681 @default.
- W2054304082 cites W2136239739 @default.
- W2054304082 cites W2150738196 @default.
- W2054304082 cites W2150764457 @default.
- W2054304082 cites W2163615816 @default.
- W2054304082 cites W2412015556 @default.
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- W2054304082 doi "https://doi.org/10.1016/j.neuro.2011.07.007" @default.
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