FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2054304826> ?p ?o ?g. }

• W2054304826 endingPage "109" @default.
• W2054304826 startingPage "104" @default.
• W2054304826 abstract "A rapid, cost-effective method for the evaluation of lower respiratory specimen has become increasingly important in the diagnosis of pulmonary diseases in immunocompromised patients. In the past, the technically demanding, time-consuming, and expensive Gomori-methenamine-silver (GMS) stain was the principal means for the evaluation of these specimens. In this study, we compared the GMS stain with a new rapid, three-stain protocol for the evaluation of lower respiratory specimens. Lower respiratory specimens were obtained by bronchoalveolar lavage (BAL). Conventional Wright/Giemsa and Gram stains were utilized, as well as a contemporary strain, calcofluor white (CW). A cell count was performed on the BAL specimens, and cytospins were stained by the three stains. The calcofluor white-stained slides were examined with an epi-fluorescent microscope, whereas the other stains were evaluated with a conventional light microscope. Gomori-methenamine-silver (GMS), acid-fast bacillus (AFB), and Papanicolaou (PAP) stains were performed as controls. Thirty-two BAL procedures were performed in 20 (63%) male patients and 12 (37%) female patients. The clinical diagnosis was pneumonia in 31% of the patients, malignant hematologic disease in 28%, acute respiratory distress syndrome (ARDS) in 9%, and acquired immunodeficiency syndrome (AIDS) in 28%. Of these specimens, 78% were adequate for interpretation and 22% were inadequate. Bacteria were found in 50% (16/32) of all BALs, fungi were found in 9% (3/32), and Pneumocystis carinii was found in 9% (3/32). Gram-positive bacteria were most frequently found in patients with pneumonia (80%, 4/5), whereas P. carinii was identified in patients with AIDS. There were no false-positive results. One CW stain was equivocal for P. carinii due to high fluorescent background. Laboratory implementation of the rapid, three-staining technique was accomplished without difficulty in microbiology and hematology laboratory sections. Specimen evaluation with the rapid staining protocol was technically easy to perform; however, experience in ultraviolet fluorescent microscopy was crucial for interpretation of CW stain. All results were available in 2 hr, cost was reduced by 30%, and the assays were available 7 days/week. Further studies are ongoing to substantiate the sensitivity, specificity, and predictive value of this technique, as well as clinical guidelines for its optimal utilization. © 1996 Wiley-Liss, Inc." @default.
• W2054304826 created "2016-06-24" @default.
• W2054304826 creator A5001215769 @default.
• W2054304826 creator A5042429828 @default.
• W2054304826 creator A5056398945 @default.
• W2054304826 creator A5090803021 @default.
• W2054304826 date "1996-01-01" @default.
• W2054304826 modified "2023-10-18" @default.
• W2054304826 title "Laboratory implementation of a rapid three-stain technique for detection of microorganisms from lower respiratory specimens" @default.
• W2054304826 cites W118518697 @default.
• W2054304826 cites W1983964290 @default.
• W2054304826 cites W1988262155 @default.
• W2054304826 cites W2034990157 @default.
• W2054304826 cites W2035978997 @default.
• W2054304826 cites W2047071865 @default.
• W2054304826 cites W2108602000 @default.
• W2054304826 cites W2143170668 @default.
• W2054304826 cites W2153846433 @default.
• W2054304826 cites W2171518705 @default.
• W2054304826 cites W2322544814 @default.
• W2054304826 cites W2325765977 @default.
• W2054304826 cites W4247624992 @default.
• W2054304826 cites W55200829 @default.
• W2054304826 doi "https://doi.org/10.1002/(sici)1098-2825(1996)10:2<104::aid-jcla8>3.0.co;2-c" @default.
• W2054304826 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8852363" @default.
• W2054304826 hasPublicationYear "1996" @default.
• W2054304826 type Work @default.
• W2054304826 sameAs 2054304826 @default.
• W2054304826 citedByCount "6" @default.
• W2054304826 countsByYear W20543048262012 @default.
• W2054304826 countsByYear W20543048262017 @default.
• W2054304826 crossrefType "journal-article" @default.
• W2054304826 hasAuthorship W2054304826A5001215769 @default.
• W2054304826 hasAuthorship W2054304826A5042429828 @default.
• W2054304826 hasAuthorship W2054304826A5056398945 @default.
• W2054304826 hasAuthorship W2054304826A5090803021 @default.
• W2054304826 hasConcept C121608353 @default.
• W2054304826 hasConcept C126322002 @default.
• W2054304826 hasConcept C141071460 @default.
• W2054304826 hasConcept C141983124 @default.
• W2054304826 hasConcept C142724271 @default.
• W2054304826 hasConcept C153460475 @default.
• W2054304826 hasConcept C154488023 @default.
• W2054304826 hasConcept C205501099 @default.
• W2054304826 hasConcept C2777559004 @default.
• W2054304826 hasConcept C2777714996 @default.
• W2054304826 hasConcept C2777914695 @default.
• W2054304826 hasConcept C2777961210 @default.
• W2054304826 hasConcept C2778220009 @default.
• W2054304826 hasConcept C2781294515 @default.
• W2054304826 hasConcept C2993443204 @default.
• W2054304826 hasConcept C501593827 @default.
• W2054304826 hasConcept C65051434 @default.
• W2054304826 hasConcept C71924100 @default.
• W2054304826 hasConcept C74864618 @default.
• W2054304826 hasConcept C86803240 @default.
• W2054304826 hasConcept C89423630 @default.
• W2054304826 hasConceptScore W2054304826C121608353 @default.
• W2054304826 hasConceptScore W2054304826C126322002 @default.
• W2054304826 hasConceptScore W2054304826C141071460 @default.
• W2054304826 hasConceptScore W2054304826C141983124 @default.
• W2054304826 hasConceptScore W2054304826C142724271 @default.
• W2054304826 hasConceptScore W2054304826C153460475 @default.
• W2054304826 hasConceptScore W2054304826C154488023 @default.
• W2054304826 hasConceptScore W2054304826C205501099 @default.
• W2054304826 hasConceptScore W2054304826C2777559004 @default.
• W2054304826 hasConceptScore W2054304826C2777714996 @default.
• W2054304826 hasConceptScore W2054304826C2777914695 @default.
• W2054304826 hasConceptScore W2054304826C2777961210 @default.
• W2054304826 hasConceptScore W2054304826C2778220009 @default.
• W2054304826 hasConceptScore W2054304826C2781294515 @default.
• W2054304826 hasConceptScore W2054304826C2993443204 @default.
• W2054304826 hasConceptScore W2054304826C501593827 @default.
• W2054304826 hasConceptScore W2054304826C65051434 @default.
• W2054304826 hasConceptScore W2054304826C71924100 @default.
• W2054304826 hasConceptScore W2054304826C74864618 @default.
• W2054304826 hasConceptScore W2054304826C86803240 @default.
• W2054304826 hasConceptScore W2054304826C89423630 @default.
• W2054304826 hasIssue "2" @default.
• W2054304826 hasLocation W20543048261 @default.
• W2054304826 hasLocation W20543048262 @default.
• W2054304826 hasOpenAccess W2054304826 @default.
• W2054304826 hasPrimaryLocation W20543048261 @default.
• W2054304826 hasRelatedWork W1502836984 @default.
• W2054304826 hasRelatedWork W2048026691 @default.
• W2054304826 hasRelatedWork W2054304826 @default.
• W2054304826 hasRelatedWork W2073814541 @default.
• W2054304826 hasRelatedWork W2106769061 @default.
• W2054304826 hasRelatedWork W2390265200 @default.
• W2054304826 hasRelatedWork W2399973971 @default.
• W2054304826 hasRelatedWork W2602741871 @default.
• W2054304826 hasRelatedWork W4254478923 @default.
• W2054304826 hasRelatedWork W1706002548 @default.
• W2054304826 hasVolume "10" @default.
• W2054304826 isParatext "false" @default.
• W2054304826 isRetracted "false" @default.
• W2054304826 magId "2054304826" @default.
• W2054304826 workType "article" @default.