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- W2054339795 abstract "A radioimmunoassay of prostagland in E2, (11α, 15-dihydroxy-9-keto-prosta-5, 13-dienoic acid; PGE2) and prostagland in F2α (9α, 11α, 15-trihydroxy-prosta-5, 13-dienoic acid; PGF2α) is described after an extraction-chromatographic separation procedure of the plasma samples. The recovery of the extraction procedure averaged respectively 98 and 97.5% while the mean recovery after extraction and chromatography was 69.8% and 80.8%. The intra-assay variation, checked by multiple analysis of a plasma pool, averaged 7.65% for PGE2 and 5.50% for PGF2α. The inter-assay variation investigated by measuring the PGE2 and PGF2α concentration in different plasma samples on two occasions, averaged 7.3% for PGE2 and 6.9% for PGF2α. The prostagland in E2 and F2α concentration increased gradually by delaying the centrifugation of the blood samples after withdrawal, probably due to release by blood platelets. Addition of a prostagland in synthesis inhibitor such as indomethacin prevented the in vitro prostaglandin synthesis as reflected by the lower (p<0.001) plasma values for PGE2 and PGF2α compared to the values obtained without indomethacin." @default.
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- W2054339795 date "2010-09-01" @default.
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- W2054339795 title "Radioimmunoassay of 11α, 15-Dihydroxy-9-Keto-Prosta-5, 13-Dienoic Acid (Prostaglandin e2) and 9α, 11α, 15-Trihydroxyprosta-5, 13-dienoic acid (prostaglandin f2α) in human plasma" @default.
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- W2054339795 doi "https://doi.org/10.1002/bscb.19810900305" @default.
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