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- W2054340556 abstract "Nuclei were isolated from light and dark grown soybean first leaves for use in studies on non-histone chromatin proteins. The ratio of DNA : RNA : protein for nuclei isolated from light grown and dark grown tissue was 1 : 0.30 : 2.81 and 1 : 0.33 : 4.02 respectively. The endogenous RNA polymerase activity of both types of nuclei was enhanced by exogenous DNA, and the addition of α-amanitin at a final concentration of 5 μg/ml resulted in 52% and 48% inhibition of RNA polymerase activity in nuclei from light and dark grown tissue respectively. The nuclei from dark grown tissue were nearly twice as active in RNA synthesis as the nuclei from light grown tissue on a DNA basis. Chromatin prepared from isolated nuclei had a 260/280 ratio if 1.63 for both light and dark grown tissue. The non-histone protein fraction of chromatin from both types of tissue was subjected to DNA-cellulose chromatography, which showed that material bound to the column and eluted at 0.6 M NaCl is capable of stimulating E. coli RNA polymerase 2–3-fold. The preparation stimulates transcription to a greater degree using native rather than denatured calf thymus DNA, and is more active at higher template concentrations. The non-histone protein fraction from soybean nuclei also stimulates purified soybean RNA polymerase II 2.5-fold when primed by soybean DNA, but to a lesser degree when primed by calf thymus DNA." @default.
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- W2054340556 date "1978-06-01" @default.
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- W2054340556 title "Transcription and RNA polymerase stimulatory activity in nuclei isolated from soybean" @default.
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- W2054340556 doi "https://doi.org/10.1016/0304-4211(78)90146-3" @default.
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