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• W2054373571 startingPage "36864" @default.
• W2054373571 abstract "Direct structural insight into the mechanisms underlying activation and desensitization remain unavailable for the pentameric ligand-gated channel family. Here, we report the structural rearrangements underlying gating transitions in membrane-embedded GLIC, a prokaryotic homologue, using site-directed spin labeling and electron paramagnetic resonance (EPR) spectroscopy. We particularly probed the conformation of pore-lining second transmembrane segment (M2) under conditions that favor the closed and the ligand-bound desensitized states. The spin label mobility, intersubunit spin-spin proximity, and the solvent-accessibility parameters in the two states clearly delineate the underlying protein motions within M2. Our results show that during activation the extracellular hydrophobic region undergoes major changes involving an outward translational movement, away from the pore axis, leading to an increase in the pore diameter, whereas the lower end of M2 remains relatively immobile. Most notably, during desensitization, the intervening polar residues in the middle of M2 move closer to form a solvent-occluded barrier and thereby reveal the location of a distinct desensitization gate. In comparison with the crystal structure of GLIC, the structural dynamics of the channel in a membrane environment suggest a more loosely packed conformation with water-accessible intrasubunit vestibules penetrating from the extracellular end all the way to the middle of M2 in the closed state. These regions have been implicated to play a major role in alcohol and drug modulation. Overall, these findings represent a key step toward understanding the fundamentals of gating mechanisms in this class of channels." @default.
• W2054373571 created "2016-06-24" @default.
• W2054373571 creator A5008484908 @default.
• W2054373571 creator A5011823547 @default.
• W2054373571 creator A5077303076 @default.
• W2054373571 date "2012-10-01" @default.
• W2054373571 modified "2023-10-13" @default.
• W2054373571 title "Conformational Transitions Underlying Pore Opening and Desensitization in Membrane-embedded Gloeobacter violaceus Ligand-gated Ion Channel (GLIC)" @default.
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• W2054373571 doi "https://doi.org/10.1074/jbc.m112.401067" @default.
• W2054373571 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3481289" @default.
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• W2054373571 hasPublicationYear "2012" @default.
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