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- W2054386457 abstract "This work reports the advantages of a label free electrochemical aptasensor for the detection of lysozyme. The biorecognition platform was obtained by the adsorption of the aptamer on the surface of a carbon paste electrode (CPE) previously blocked with mouse immunoglobulin under controlled-potential conditions. The recognition event was detected from the decrease in the guanine and adenine electro-oxidation signals produced as a consequence of the molecular interaction between the aptamer and lysozyme. The biosensing platform demonstrated to be highly selective even in the presence of large excess (9-fold) of bovine serum albumin, cytochrome C and myoglobin. The reproducibility for 10 repetitive determinations of 10.0 mg L−1 lysozyme solution was 5.1% and 6.8% for guanine and adenine electro-oxidation signals, respectively. The detection limits of the aptasensor were 36.0 nmol L−1 (if considering guanine signal) and 18.0 nmol L−1 (if taking adenine oxidation current). This new sensing approach represents an interesting and promising alternative for the electrochemical quantification of lysozyme." @default.
- W2054386457 created "2016-06-24" @default.
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- W2054386457 date "2009-04-15" @default.
- W2054386457 modified "2023-10-10" @default.
- W2054386457 title "Label-free electrochemical aptasensor for the detection of lysozyme" @default.
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- W2054386457 doi "https://doi.org/10.1016/j.talanta.2008.11.002" @default.
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