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- W2054391652 abstract "Digital holographic microscopy (DHM) is a technique that allows obtaining, from a single recorded hologram, quantitative phase image of living cell with interferometric accuracy. Specifically the optical phase shift induced by the specimen on the transmitted wave front can be regarded as a powerful endogenous contrast agent, depending on both the thickness and the refractive index of the sample. We have recently proposed a new and efficient decoupling procedure allowing to directly obtain separate measurements of the thickness and the integral refractive index of a given living cell. Consequently, it has been possible to accurately measure (with a precision of 0.0003) the mean refractive index and the volume of living erythrocytes. Here, application of this decoupling procedure on erythrocyte allows to measure a refractive index of 1.40 and a mean volume of about 106 μm<sup>3</sup>." @default.
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- W2054391652 date "2007-02-08" @default.
- W2054391652 modified "2023-10-03" @default.
- W2054391652 title "Erythrocytes volume and refractive index measurement with a digital holographic microscope" @default.
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- W2054391652 doi "https://doi.org/10.1117/12.700463" @default.
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